IGEM:Imperial/2010/Fast Response module
|2 components systems|
The first idea, that seems to be feasible considering all the components, is the one below:
This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.
- The split protease TEV: Monitoring regulated protein-protein interactions using split TEV
- The EnvZ-OmpR system: The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.