IGEM:Imperial/2010/Fast Response module - Revision history

Benjamin Miller at 16:11, 23 September 2010

Benjamin Miller — Thu, 23 Sep 2010 16:11:50 GMT

←Older revision		Revision as of 16:11, 23 September 2010
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		+	{{Imperial2010/Header}}
	{\| class="wikitable" style="text-align: center; width: 20%; height: 170px;" border="1"		{\| class="wikitable" style="text-align: center; width: 20%; height: 170px;" border="1"

Nicolas Kylilis — Fri, 23 Jul 2010 15:40:06 GMT

←Older revision		Revision as of 15:40, 23 July 2010
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	*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641 The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]		*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641 The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]
		+
		+	*all about EnvZ [http://www.bio.cmu.edu/courses/03441/termpapers/99TermPapers/TwoCom/envz_details.html]
		+
		+	*all about OmpR [http://www.bio.cmu.edu/courses/03441/termpapers/99TermPapers/TwoCom/ompr_details.html]

Nicolas Kylilis — Tue, 20 Jul 2010 09:54:38 GMT

Idea 1

←Older revision		Revision as of 09:54, 20 July 2010
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	References:		References:

-	*The split protease TEV: [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html\| Monitoring regulated protein-protein interactions using split TEV]	+	*The split protease TEV: [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html Monitoring regulated protein-protein interactions using split TEV]

-	*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641\|The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]	+	*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641 The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]

Nicolas Kylilis — Tue, 20 Jul 2010 09:51:56 GMT

Idea 1

←Older revision		Revision as of 09:51, 20 July 2010
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	References:		References:

-	*The split protease TEV: [[http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html\|Monitoring regulated protein-protein interactions using split TEV]]	+	*The split protease TEV: [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html\| Monitoring regulated protein-protein interactions using split TEV]

-	*The EnvZ-OmpR system: [[http://www.ncbi.nlm.nih.gov/pubmed/15979641\|The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]]	+	*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641\|The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]

Nicolas Kylilis — Tue, 20 Jul 2010 09:42:52 GMT

Idea 1

←Older revision		Revision as of 09:42, 20 July 2010
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	==Idea 1==		==Idea 1==
	The first idea, that seems to be feasible considering all the components, is the one below:		The first idea, that seems to be feasible considering all the components, is the one below:
		+
		+	[[Image:OmpR system pic.jpg]]
		+
		+	This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.
		+
		+
		+	References:
		+
		+	*The split protease TEV: [[http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html\|Monitoring regulated protein-protein interactions using split TEV]]
		+
		+	*The EnvZ-OmpR system: [[http://www.ncbi.nlm.nih.gov/pubmed/15979641\|The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]]

Nicolas Kylilis — Tue, 20 Jul 2010 09:21:18 GMT

Idea 1

←Older revision		Revision as of 09:21, 20 July 2010
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	==Idea 1==		==Idea 1==
-	The first idea that seems to be feasible considering all the components is the one below:	+	The first idea, that seems to be feasible considering all the components, is the one below:

Nicolas Kylilis — Mon, 19 Jul 2010 16:02:10 GMT

←Older revision		Revision as of 16:02, 19 July 2010
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	\| Vibrio cholera		\| Vibrio cholera
	\|}		\|}
		+
		+	==Idea 1==
		+	The first idea that seems to be feasible considering all the components is the one below:

Piotr D. Faba — Mon, 19 Jul 2010 11:42:06 GMT

Rearranging signalling into Fast response page and removing effectors into Output page

←Older revision		Revision as of 11:42, 19 July 2010
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-	{\| class="wikitable" style="text-align: center; width: 50%; height: 170px;" border="1"	+	{\| class="wikitable" style="text-align: center; width: 20%; height: 170px;" border="1"

-	\|+ ~~Table of ideas~~	+	\|+ Ideas
	\|-		\|-
-	! ~~Effector 1 (Protease) !! Effector 2 (Dye,Enz)~~	+	! Signalling
	\|-		\|-
-	\| [[IGEM:Imperial/2010/~~Fast_Response_module~~/~~transcr_sigma54~~ \| ~~transcr sigma 54~~]] ~~\|\| 2 colourless~~	+	\| [[IGEM:Imperial/2010/Detection_module/2_components_systems \| 2 components systems]]
	\|-		\|-
-	\| ~~[[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation\|Activation (phosphorylation)]]~~	+	\| logic gate
-	~~\| Enz-in-pathway~~	+
-		+
-	* short pathways	+
-	* ensure - colourless -> colour	+
	\|-		\|-
-	\| ~~[[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases \| Target proteases ]] to use: Piotr, Nick, Kyasha~~	+	\| Vibrio cholera
-	* not many AA	+
-	* non-toxic	+
-	* can work in E.Coli	+
-	* quantize speed, efficiency	+
-	~~\| Protein scaffold: Maddie~~	+
-		+
-	\|-	+
-	~~\| 2C DNA binding prot release \|\|~~	+
	\|}		\|}
-
-	~~James- some suggested papers:~~
-	~~<biblio>~~
-	~~#1 pmid=17117150~~
-	~~#2 pmid=17072307~~
-	~~</biblio>~~

Nicolas Kylilis — Thu, 15 Jul 2010 16:06:45 GMT

creation of an activation page

←Older revision		Revision as of 16:06, 15 July 2010
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	\| [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 \| transcr sigma 54]] \|\| 2 colourless		\| [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 \| transcr sigma 54]] \|\| 2 colourless
	\|-		\|-
-	\| Activation (phosphorylation)	+	\| [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation\|Activation (phosphorylation)]]
	\| Enz-in-pathway		\| Enz-in-pathway

James Chappell — Thu, 15 Jul 2010 09:12:51 GMT