IGEM:Imperial/2010/Fast Response module

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Current revision (11:11, 23 September 2010) (view source)
 
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{| class="wikitable" style="text-align: center; width: 50%; height: 170px;" border="1"
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{{Imperial2010/Header}}
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{| class="wikitable" style="text-align: center; width: 20%; height: 170px;" border="1"
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|+ Table of ideas
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|+ Ideas
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   ! Effector 1 (Protease) !! Effector 2  (Dye,Enz)
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   ! Signalling
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   | [[IGEM:Imperial/2010/Calendar/2010/07/13/transcr sigma54 | transcr sigma 54]] || 2 colourless
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   | [[IGEM:Imperial/2010/Detection_module/2_components_systems | 2 components systems]]
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   | Activation (phosphorylation)
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   | logic gate
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  | Enz-in-pathway
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* short pathways
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* ensure - colourless -> colour
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   | [[IGEM:Imperial/2010/Target_Proteases | Target proteases ]] to use: Piotr, Nick, Kyasha
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   | Vibrio cholera
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* not many AA
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* non-toxic
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* can work in E.Coli
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* quantize speed, efficiency
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  | Protein scaffold: Maddie
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  | 2C DNA binding prot release ||
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==Idea 1==
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The first idea, that seems to be feasible considering all the components, is the one below:
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[[Image:OmpR system pic.jpg]]
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This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.
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References:
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*The split protease TEV: [http://www.nature.com/nmeth/journal/v3/n12/full/nmeth967.html Monitoring regulated protein-protein interactions using split TEV]
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*The EnvZ-OmpR system: [http://www.ncbi.nlm.nih.gov/pubmed/15979641 The response regulator OmpR oligomerizes via beta-sheets to form head-to-head dimers.]
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*all about EnvZ [http://www.bio.cmu.edu/courses/03441/termpapers/99TermPapers/TwoCom/envz_details.html]
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*all about OmpR [http://www.bio.cmu.edu/courses/03441/termpapers/99TermPapers/TwoCom/ompr_details.html]

Current revision

Ideas
Signalling
2 components systems
logic gate
Vibrio cholera

Idea 1

The first idea, that seems to be feasible considering all the components, is the one below:

Image:OmpR system pic.jpg

This is a bacterial two component system. On recieving of a signal, the Env Z receptor autophosphorylates. The phosphate is then passed on the respond element, a transcription factor called OmpR. OmpR is a molecule found naturally downstream of the signalling cascade from the EnvZ receptor. On phosphorylation the OmpR-P dimerise. Since each OmpR is fused with a split protease, when the OmpRs come together, the split protease (TEV) domains come together. This produces the active protease.


References:

  • all about EnvZ [1]
  • all about OmpR [2]
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