IGEM:Imperial/2010/Fast Response module

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   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless
   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless
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|-
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   | Activation (phosphorylation)  
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   | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]]
   | Enz-in-pathway  
   | Enz-in-pathway  

Revision as of 12:06, 15 July 2010

Table of ideas
Effector 1 (Protease) Effector 2 (Dye,Enz)
transcr sigma 54 2 colourless
Activation (phosphorylation) Enz-in-pathway
  • short pathways
  • ensure - colourless -> colour
Target proteases to use: Piotr, Nick, Kyasha
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold: Maddie
2C DNA binding prot release

James- some suggested papers:

  1. Kerppola TK. . pmid:17117150. PubMed HubMed [1]
  2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. . pmid:17072307. PubMed HubMed [2]
All Medline abstracts: PubMed HubMed
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