IGEM:IMPERIAL/2009/phases of project

Phase 1: Protein production

Time regulation

1. NYMU 2008 Project

2. Genetic toggle switch
File:Timer-mutually repressive

3. Timer from Dr. Karen's talk
File:Timer-dr karen

Phase 2: Encapsulation

Cell Encapsulation

why encapsulate?

• enhances cell resistance to freezing and freeze-drying (for storage)
• added convenience and reduced packaging costs
• longer stability and viability during storage

natural encapsulation

encapsulation occurs naturally when bacteria trapped in own exo-secretions many lactobacilli secrete exo-polysaccharides, but most are insufficient to cover fully

Recent review on probiotic encapsulation methods

Phase 3: Plasmid deletion

• Bactoblood Project from Berkly 2007 - Plasmid Deletion and Freeze Drying Bacteria
• Programmed Cell Death - Use of anti-toxins and toxins to control cell death
• Cell Death mechanism by KU Leaven 2008 iGEM
• Articles about using restriction enzymes to lyse the host chromosome
• Class of Bacterial toxin