IGEM:IMPERIAL/2009/Assays Protocols

Current Wet Lab Plan

Step By Step Wet-Lab Plan

Assays

Module 1

Cellulase

Cellulase Assay from Sigma Aldrich
Cellulase Assay (Sigma)   Quantitative

Alternative Cellulase Assay
Cellulase Assay

• Charles Fracchia 06:16, 4 August 2009 (EDT): Dead link above

Feedback - In vitro or vivo? Do we need to purify enzyme for assay? presumably so, as requires measurement of glucose concentation, therefore not good to use cell extract

Phenylalanine Hydroxylase (PAH)

Phenylalanine Hydroxylase Assay from Sigma Aldrich :
PAH Assay   Quantitative

Module 2

Colanic Acid Assay

Trehalose Assay

Module 3

Heat induction Assays

Cell Death Assays

Cloning Strategies

Cloning Strategy for Module 1 and Timer

Cloning Strategy for Module 2

Testing Constructs

Testing Constructs for all modules