IGEM:IMPERIAL/2009/Assays Protocols

Assays

Module 1

Cellulase

Cellulase Assay from Sigma Aldrich
Cellulase Assay (Sigma)

Alternative Cellulase Assay
| Cellulase Assay

Feedback - In vitro or vivo? Do we need to purify enzyme for assay? presumably so, as requires measurement of glucose concentation, therefore not good to use cell extract

Phenylalanine Hydroxylase (PAH)

Phenylalanine Hydroxylase Assay from Sigma Aldrich :
PAH Assay

Module 2

Colanic Acid Assay

Trehalose Assay

Module 3

Cell Death Assays

Cloning Strategies

Cloning Strategy for Module 1 and Timer

Cloning Strategy for Module 2

Step By Step Wet-Lab Plan