IGEM:IMPERIAL/2009/Assays Protocols: Difference between revisions
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=== Phenylalanine Hydroxylase (PAH)=== | === Phenylalanine Hydroxylase (PAH)=== | ||
Phenylalanine Hydroxylase Assay from Sigma Aldrich : <br> | Phenylalanine Hydroxylase Assay from Sigma Aldrich : <br> | ||
[[Media:PAH Assay.pdf | PAH Assay]] | [[Media:PAH Assay.pdf | PAH Assay]] <b>Quantitative</b> | ||
==Module 2== | ==Module 2== | ||
Revision as of 03:18, 4 August 2009
Assays
Module 1
Cellulase
Cellulase Assay from Sigma Aldrich
Cellulase Assay (Sigma) Quantitative
Alternative Cellulase Assay
Cellulase Assay
- Charles Fracchia 06:16, 4 August 2009 (EDT): Dead link above
Feedback - In vitro or vivo? Do we need to purify enzyme for assay? presumably so, as requires measurement of glucose concentation, therefore not good to use cell extract
Phenylalanine Hydroxylase (PAH)
Phenylalanine Hydroxylase Assay from Sigma Aldrich :
PAH Assay Quantitative
Module 2
Module 3
Cloning Strategies
Cloning Strategy for Module 1 and Timer
