IGEM:IMPERIAL/2009/4Sep/System Overview/Mod1: Difference between revisions
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* Number of cloning steps remaining before testing construct | * Number of cloning steps remaining before testing construct | ||
* Gene Synthesis involved ? (if yes, when is it due ?) | * Gene Synthesis involved ? (if yes, when is it due ?) | ||
Yes. Due date up to gene art, probably by next week. | |||
* How likely are we to get the cloning finished before [Jamboree - 2 weeks] (deadline to send parts) ? [unlikely, likely, certain] | * How likely are we to get the cloning finished before [Jamboree - 2 weeks] (deadline to send parts) ? [unlikely, likely, certain] | ||
Likely | |||
===Data we hope to Get from this=== | ===Data we hope to Get from this=== | ||
Revision as of 07:23, 4 September 2009
Module 1
Module function in overall design
- High level description:
This phase is for protein production. Our two proteins of interest are
1) PAH
2) Cellulase
Production is initially repressed by LacI. When IPTG is added, derepression occurs, protein production starts.
- Input(s)/Output(s):
Input: IPTG.
Output: Protein of interest.
HIGH IPTG = Production of protein LOW IPTG = Protein production repressed
Assays to be done
| Assays | Importance (1=Most important. 3=Least important) | When? (1=Now. 3=Last) | Variable | Rationale | Output | Model |
| LacI IPTG (expression) | 2 | 2 | IPTG | Influence of IPTG on protein production & cell growth | IPTG | Yes |
| Cellulase (activity) | 2 | 3 | - | - | - | MM |
| PAH (activity) | 2 | 3 | IPTG | - | - | MM |
| Cellulase concentration | 3 | 3 | - | - | - | MM |
| PAH concentration | 3 | 3 | - | - | - | MM |
Testing Construct Design
- How many of them ?
2 of them. 1 for PAH, 1 for cellulase
- Picture/Diagram for all test constructs
- Describe briefly purpose behind each test construct
For both cellulase and PAH, RFP fluorescence is the output. This allows us to determine if cellulase and PAH are being produced from respective constructs.
Progress
- Current status in terms of cloning for each assembly
- Number of cloning steps remaining before testing construct
- Gene Synthesis involved ? (if yes, when is it due ?)
Yes. Due date up to gene art, probably by next week.
- How likely are we to get the cloning finished before [Jamboree - 2 weeks] (deadline to send parts) ? [unlikely, likely, certain]
Likely
Data we hope to Get from this
- Fore each test construct, sketch graph or write description, but make sure it is clear and understanable
- How essential is this data for the success of the project (0..10)
Ready for testing construct
- Protocols written and reviewed ?
1) PAH - test kit to be bought
2) Cellulase - test kit to be bought
- Any expertise needed? Acces to specific equipment needed ?
- Modelling done ? Ready to analyse experimental data ?
Yes. Link to M1 modelling page
Comments
- Additional info you think is important.
