IGEM:IMPERIAL/2008/Prototype/Light: Difference between revisions
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Photoreceptors and domains that were considered in our design include rhodopsins, phytochromes, LOV and BLUF. Of these, ''B. subtilis'' contains the blue light sensing protein YtvA which is related to the plant blue-light receptors phototropins. YtvA is a positive activator of the general stress transcription factor σB. Blue and not red light activates YtvA and thus induces σB activity. We could use σB which is expressed upon blue light illumination to activate the production of EpsE and hence control bacteria motility. However, there are other [[IGEM:IMPERIAL/2008/Bioprinter/Stress_response| stressors]] which activate σB, hence we aim to make blue light the main activator of σB by controlling growth conditions and over expressing the YtvA receptor. | Photoreceptors and domains that were considered in our design include rhodopsins, phytochromes, LOV and BLUF. Of these, ''B. subtilis'' contains the blue light sensing protein YtvA which is related to the plant blue-light receptors phototropins. YtvA is a positive activator of the general stress transcription factor σB. Blue and not red light activates YtvA and thus induces σB activity. We could use σB which is expressed upon blue light illumination to activate the production of EpsE and hence control bacteria motility. However, there are other [[IGEM:IMPERIAL/2008/Bioprinter/Stress_response| stressors]] which activate σB, hence we aim to make blue light the main activator of σB by controlling growth conditions and over expressing the YtvA receptor. | ||
|[[Image:YtvA_Pathway.jpg|300px|The YtvA-SigmaB activation pathway]] | |[[Image:YtvA_Pathway.jpg|300px|The YtvA-SigmaB activation pathway]] | ||
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Revision as of 07:22, 28 October 2008
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