IGEM:IMPERIAL/2008/New/Protocols

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Home	Biofabricator Subtilis Project Specifications Why B. subtilis? B. subtilis: Benefits vs Challenges	Wet Lab Wet Lab Hub Cloning Strategy Experiments & Protocols Experimental Results BioBricks & Characterisation	Dry Lab Dry Lab Hub Growth Curves Genetic Circuits Motility Analysis Appendices - Code etc.	Notebook	Our Team

Protocols

1. Transformation Protocols for integration vectors in Bacillus subtilis
Protocol 1 Protocol 2

2. Transformation Protocol for linear DNA in Bacillus subtilis

3. Transformation Protocol E.coli

Preparation of XL1-blue electrocompetent cells

4. Motility Analysis

Motility Assays

5. PCR

6. Calibration Curves

7. Promoter-RBS Testing Protocols

8. Biomaterial Protocols

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