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= Protocols =
 
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{{Imperial/Box2|Experiments & Protocols|
Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for ''B. subtilis'' manipulation explicitly, as other teams have previously had difficulty in this area.
 
These protocols worked for us, so any future teams looking to use ''subtilis'' could save quite a bit of time by looking here before diving into the literature!}}
 
 
{| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="center" width=50%
|width="100%"| <font size=4px color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</font>
|width="100%"| <font size=4px color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</font>
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{{Imperial/EndPage|Cloning_Strategy|Major_Results}}
{{Imperial/EndPage|Cloning_Strategy|Major_Results}}

Latest revision as of 05:20, 30 September 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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Experiments & Protocols

Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for B. subtilis manipulation explicitly, as other teams have previously had difficulty in this area.

These protocols worked for us, so any future teams looking to use subtilis could save quite a bit of time by looking here before diving into the literature!



1. Transformation Protocols for integration vectors in Bacillus subtilis
2. Transformation Protocol for linear DNA in Bacillus subtilis
  • Protocol
3. Transformation Protocol E.coli
4. Motility Analysis
5. PCR
6. Calibration Curves
7. Promoter-RBS Testing Protocols
8. Biomaterial Protocols
  • Protocol



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