IGEM:IMPERIAL/2008/New/Protocols

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= Protocols =
= Protocols =
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{{Protocols}}
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|width="100%"| <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''
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*[[/Transformation_protocol_2 | Protocol 1]]
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*[[/Transformation_protocol_4 | Protocol 2]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''
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*Protocol
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|style="background:#66aadd"| <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
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*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''4. Motility Analysis'''
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*[[/Protocols#Motility_Experiments | Motility Assays]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''5. PCR'''
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*[[/PCR| PCR]]
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*[[/Colony_PCR| Single Colony PCR]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''6. Calibration Curves'''
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*[[/Calibration-Curves| Calibration Curves ]]
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*[[/Growth| O.D.<sub>600</sub> vs cell number]]
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*[[/Fluorescence| Fluorescence vs GFPmut3b]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''
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*[[/Constitutive | Constitutive Promoters and RBS]]
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*[[/Inducible | pHyperspank Promoter and RBS]]
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*[[/Light_Inducible | Light Inducible Promoters and RBS]]
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|style="background:#66aadd"| <font size=4px><font color=white>'''8. Biomaterial Protocols'''
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*Protocol
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<table><tr><td>
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{{Imperial/EndPage|Cloning_Strategy|Major_Results}}
{{Imperial/EndPage|Cloning_Strategy|Major_Results}}

Revision as of 09:43, 18 September 2008




Protocols

In vivo

Escherichia coli

{{{ecoli}}}

Yeast

{{{yeast}}}

T7

{{{T7}}}

Mesoplasma florum

{{{meso}}}

In vitro

Nucleic acids

{{{nucleic acids}}}

DNA

{{{DNA}}}

RNA

{{{RNA}}}

Protein

{{{Protein}}}

Other

Microfluidics

{{{microfluidics}}}

Microscopy

{{{microscopy}}}

Flow Cytometry

{{{cytometry}}}

Plate reader

{{{plate reader}}}



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