IGEM:IMPERIAL/2008/New/Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Protocols)
m (Protocols)
Line 2: Line 2:
= Protocols =
= Protocols =
-
{|
+
 
-
|Little
+
{| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50%
-
|Table
+
|width="100%"| <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''
|-
|-
-
|colspan="2" |With rows
+
|style="background:#FFFFFF"|
 +
*[[/Transformation_protocol_2 | Protocol 1]]
 +
*[[/Transformation_protocol_4 | Protocol 2]]
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''
 +
|-
 +
|style="background:#FFFFFF"|
 +
*Protocol
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
 +
|-
 +
|style="background:#FFFFFF"|
 +
*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''4. Motility Analysis'''
 +
|-
 +
|style="background:#FFFFFF"|
 +
*[[/Protocols#Motility_Experiments | Motility Assays]]
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''5. PCR'''
 +
|-
 +
|style="background:#FFFFFF"|
 +
*[[/PCR| PCR]]
 +
*[[/Colony_PCR| Single Colony PCR]]
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''6. Calibration Curves'''
 +
|-
 +
|style="background:#FFFFFF"|
 +
*[[/Calibration-Curves| Calibration Curves ]]
 +
*[[/Growth| O.D.<sub>600</sub> vs cell number]]
 +
*[[/Fluorescence| Fluorescence vs GFPmut3b]]
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''
 +
|-
 +
|style="background:#FFFFFF"|
 +
*[[/Constitutive | Constitutive Promoters and RBS]]
 +
*[[/Inducible | pHyperspank Promoter and RBS]]
 +
*[[/Light_Inducible | Light Inducible Promoters and RBS]]
 +
|-
 +
|style="background:#66aadd"| <font size=4px><font color=white>'''8. Biomaterial Protocols'''
 +
|-
 +
|style="background:#FFFFFF"|  
 +
*Protocol
|}
|}
{{Imperial/EndPage|Cloning_Strategy|Major_Results}}
{{Imperial/EndPage|Cloning_Strategy|Major_Results}}

Revision as of 08:33, 18 September 2008




Protocols

1. Transformation Protocols for integration vectors in Bacillus subtilis
2. Transformation Protocol for linear DNA in Bacillus subtilis
  • Protocol
3. Transformation Protocol E.coli
4. Motility Analysis
5. PCR
6. Calibration Curves
7. Promoter-RBS Testing Protocols
8. Biomaterial Protocols
  • Protocol


</div></td></tr></table>

Personal tools