Revision as of 18:14, 11 September 2008


Home	Biofabricator Subtilis Project Specifications Why B. subtilis? B. subtilis: Benefits vs Challenges	Wet Lab Wet Lab Hub Cloning Strategy Experiments & Protocols Experimental Results BioBricks & Characterisation	Dry Lab Dry Lab Hub Growth Curves Genetic Circuits Motility Analysis Appendices - Code etc.	Notebook	Our Team

Protocols

1. Transformation Protocols for integration vectors in Bacillus subtilis
Protocol 1 Protocol 2
2. Transformation Protocol for linear DNA in Bacillus subtilis
Protocol
3. Transformation Protocol E.coli
Preparation of XL1-blue electrocompetent cells
4. Motility Analysis
Motility Assays
5. PCR
PCR Single Colony PCR
6. Calibration Curves
Calibration Curves O.D.₆₀₀ vs cell number Fluorescence vs GFPmut3b
7. Promoter-RBS Testing Protocols
Constitutive Promoters and RBS pHyperspank Promoter and RBS Light Inducible Promoters and RBS
8. Biomaterial Protocols

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