IGEM:IMPERIAL/2008/New/Project

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<html><a href=http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype><img width=50px src=http://openwetware.org/images/f/f2/Imperial_2008_Logo.png></img</a></html> Home The Project B.subtilis Chassis Wet Lab Dry Lab Notebook

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Authors: Wiki team

Editors: Wiki team

Project

In designing our bioprinter, we followed the engineering cycle. Thus we start with basic specifications our project needs to meet to fulfil our aims...

Specifications

Controllable motility (motility)

    • Quick and effective control (stopping, specific time?)
    • Reversible - not necessarily as important as stopping, but would be good for it to be able to start again

[Picture]

Ability to respond to outside stimuli (light)

    • Must have a reasonable (specific?) response time
    • Must be able to be coupled to an inducible promoter
    • Detectable be compatible with the chassis and be able to be over-expressable to increase efficiency
    • Must have minimal negative secondary effects

[Picture]

Biomaterial synthesis and excretion (biomaterials)

    • Should be able to effectively secrete detectable amounts of peptides into the growth medium.
    • Self-assembly outside the chassis to form a 3D structure
    • Small peptide for easy excretion

[Picture]



Please continue on to see how these specifications are met by B. subtilis, and hence why we chose the ambitious route of using it as our chassis! > The Chassis Page >



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