IGEM:IMPERIAL/2008/New/Motility/Validation: Difference between revisions
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=Validating Tracking Software= | = Validating Tracking Software = | ||
ImageJ was used primarily to analyse images. Various plugins were tested, such as SpotTracker and Particle Tracker, but both failed to producing any form of convincing result. Microscopy videos were captured using Volocity software, which also provided a cell tracking function. Thus analysis of synthetic videos was done '''manually''' and using 64-Bit '''Volocity''' analysis software. The synthetic video shown to the right was used as an input for the tracking of synthetic cells. | ImageJ was used primarily to analyse images. Various plugins were tested, such as SpotTracker and Particle Tracker, but both failed to producing any form of convincing result. Microscopy videos were captured using Volocity software, which also provided a cell tracking function. Thus analysis of synthetic videos was done '''manually''' and using 64-Bit '''Volocity''' analysis software. The synthetic video shown to the right was used as an input for the tracking of synthetic cells. | ||
==Volocity== | == Volocity == | ||
Volocity 64-Bit was used to analyse synthetic videos created. The following protocol was followed: | Volocity 64-Bit was used to analyse synthetic videos created. The following protocol was followed: | ||
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#Begin tracking using Ctrl-U | #Begin tracking using Ctrl-U | ||
==Manual Tracking== | == Manual Tracking == | ||
Synthetic cells were tracked using ImageJ via the Manual Tracking Plugin. The following protocol was followed: | Synthetic cells were tracked using ImageJ via the Manual Tracking Plugin. The following protocol was followed: | ||
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#Once tracking is complete, end track and save results with a .xls extension. | #Once tracking is complete, end track and save results with a .xls extension. | ||
=Validation Process= | = Validation Process = | ||
A synthetic video of five cells with changing shape was used in the evaluation of tracking software. Each cell was tracked using respective tracking software, and their coordinates in pixels were obtained. | A synthetic video of five cells with changing shape was used in the evaluation of tracking software. Each cell was tracked using respective tracking software, and their coordinates in pixels were obtained. | ||
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The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels. | The errors associated with manual tracking and Volocity software are shown below. All errors are given in terms of pixels. | ||
==Manual Tracking== | == Manual Tracking == | ||
{| border="1" cellpadding="5" cellspacing="0" align="center" | {| border="1" cellpadding="5" cellspacing="0" align="center" | ||
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==Volocity== | == Volocity == | ||
{| border="1" cellpadding="5" cellspacing="0" align="center" | {| border="1" cellpadding="5" cellspacing="0" align="center" | ||
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=Evaluation= | = Evaluation = | ||
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==Conclusion== | == Conclusion == | ||
*Manual tracking will be used to track ''B. subtilis''. | *Manual tracking will be used to track ''B. subtilis''. | ||
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Latest revision as of 13:01, 16 September 2008
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