IGEM:IMPERIAL/2008/New/Home

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<html><a href=http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype><img width=50px src=http://openwetware.org/images/f/f2/Imperial_2008_Logo.png></img</a></html> Home The Project B.subtilis Chassis Wet Lab Dry Lab Notebook

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New Wiki Layout - read and ask Tom if you have any questions!

Overall editing will be done by Tom and Erika when everything is uploaded. On top of everything will be a home page (this page), which will basically act as a front cover for our project - a nice cartoon, something flashy and impressive, leading on the the project page.

Basically please note from the map below which pages you are responsible for. When we get to updating the wiki (to put it on the official one) we'll each need to summarise our pages and put them up, fixing any links and re-uploading any images/files so they point to the right place! If you have any problems or are unsure of how to do something, ask Tom or QQ. By the end of week 10, please could everyone have a relevant summary of their work - along with necessary diagrams and results - up on these pages so we can see what we've got.

Key: Small letters are teams. Wiki team (i) is Tom and QQ, drylab team (d) is Erika, Clinton, Prudence and Yanis and the wetlab team (w) is James, Chris, Tom, Krupa and QQ. Large letters are names (first name initials, except Clinton is L).



Keep working and putting things on the "Prototype" wiki, and use this one for finished work and so on. Try not to make new pages - we're aiming for a small wiki, concisely written but with all the necessary information. Remember to ask us (or bring it up at a debrief!) if you're having trouble, think it could be organised better a different way, or anything else.

~Tom and QQ



Welcome to the Imperial 2008 iGEM team's main project page. It's Friday, April 19 and a great day to read about an awesome iGEM project!


For the 2008 iGEM competition, the Imperial College team is working on the foundations for a biofabricator. We are using the Gram-positive Bacillus subtilis bacterium as our chassis (for a variety of reasons) and hope to exert fine control over its movement via a recently-discovered clutch mechanism [1]. Using light as a stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set 3D pattern. The project was inspired by 3D printers used in rapid prototyping, and our "blue-sky" aim is to design a full 3D biofabrication machine!
The Team Prudence is taking the picture... Click for larger image!
The Imperial College 2008 iGEM team consists of nine students (five bioengineers, three biochemists and one biologist), five advisors and two professors. You can find out more about the team members at the team page.


Overview of our planned system Basic Circuit Diagram This diagram gives a basic overview of how we intend our system to work. In the starting phase, B. subtilis are motile and are not producing our desired product - they swim freely in the medium. If we want to construct a bio-scaffold with an "I" shape, we shine light of the correct wavelength (red is used as an arbitrary example here) in the desired shape onto the plate.

Bacteria within this area will sense that light, and production of a clutch molecule will be triggered. This disengages the flagella from the motor quite quickly, rendering the subtilis stationary.

Coupled with the clutch is a gene for expression of peptides for our bio-scaffold material, so they will start producing them when in the area. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop. A basic circuit overview of how we hope to achieve this is shown. We had also considered engineering B. subtilis to release a chemoattractant to bring in "reinforcements" and improve localisation, but this may be beyond the scope of our project.

3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabricator.


Test page and storage for random parts - Also see here for intro to editing the Imperial Wiki

Revisit the original Wiki layout

Imperial College's iGEM team 2008 would like to thank our sponsors: <html><center><a href=http://www.bio-rad.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/BioRad.png></a><a href=http://www.fisher.co.uk/><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/FisherScientific.jpg></a><a href=http://www.geneart.com/><img src=http://i59.photobucket.com/albums/g305/Timpski/GeneArt.gif></a><a href=http://www.vwr.com/index.htm><img height=50px src=http://i59.photobucket.com/albums/g305/Timpski/VWR.jpg></a></center></html>


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