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= New Wiki Layout - read and ask Tom if you have any questions! =
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{| cellpadding="1" style="background:#2B48B3; border:4px solid #99BBFF; color:#E5EBFF" align="center" width=90%
|<center>Welcome to the Imperial 2008 iGEM project page. It's {{CURRENTDAYNAME}}, {{CURRENTMONTHNAME}} {{CURRENTDAY}} and a great day to read about an awesome iGEM project!</center>
|}
<br>
{{Imperial/Box1|<html><center><img width="350px" src="http://i59.photobucket.com/albums/g305/Timpski/Logo1.png"></center></html>
|For the 2008 iGEM competition, the Imperial College Team aims to develop a genetically-engineered Biofabricator, using the Gram-positive bacterium Bacillus subtilis as our chassis. Our Biofabricator aims to produce self-assembling biomaterials in specified 3D shapes, using light as the trigger.
<br><br>
This is achieved in three stages. First by utilising an endogenous light-sensing mechanism, the bacteria is captured in the desired location using 3D holography. Next bacterial locomotion is suspended in the region of interest using a recently-discovered clutch mechanism. This involves disengaging the flagellum from the motor protein. Finally, when our bacteria are stationary in the correct location, the biomaterial production is triggered. These biomaterials can self-assemble to form a 3D bio-scaffold. Applications of our Biofabricator range from regenerative tissue engineering to Bio-Couture.
<br><br>
[[Image:Imperial_2008_Bioprinter_Cartoon.png |center|600px| Overview of our planned system]]
[[Image:Imperial_2008_Basic_Circuit.jpg |center|Basic Circuit Diagram]]
<br><br>
Please continue on to our project pages - you may want to start with our [[IGEM:IMPERIAL/2008/New/Project| '''>>> Project Specifications >>>''']]}}
<hr>
{{Imperial/Box1||
The Imperial College Team 2008 has received sponsorship from a number of generous companies. We are grateful for their kind support.
<html><center><a href="http://www.bio-rad.com/"><img height="40px" src="http://i59.photobucket.com/albums/g305/Timpski/Biorad.png"></a><a href="http://www.fisher.co.uk/"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/Fisher.png"></a><a href="http://www.geneart.com/"><img height="25px" src="http://i59.photobucket.com/albums/g305/Timpski/Geneart.png"></a><a href="http://www.vwr.com/index.htm"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/VWR.png"></a></center></html>
}}


Overall editing will be done by Tom and Erika when everything is uploaded. On top of everything will be a home page (this page), which will basically act as a front cover for our project - a nice cartoon, something flashy and impressive, leading on the the project page.
{{Imperial/EndPage|Home|Project}}
 
Key: Small letters are teams. W'''i'''ki team (i) is Tom and QQ, '''d'''rylab team (d) is Erika, Clinton, Prudence and Yanis and the '''w'''etlab team (w) is James, Chris, Tom, Krupa and QQ.
Large letters are names (first name initials, except Clinton is '''L''').
 
 
*[[IGEM:IMPERIAL/2008/New/Project | Project]] (Specifications) [i]
**[[IGEM:IMPERIAL/2008/New/Team | Team]] [i, K]
**Notebook
***Calendar QQ - editor
***Minor Results [w]
**[[IGEM:IMPERIAL/2008/New/Chassis_1 | Chassis 1]] (How specs are met by ''subtilis'') [i]
***[[IGEM:IMPERIAL/2008/New/Chassis_2 | Chassis 2]] (Pros and cons, other information) [C, J]
**[[IGEM:IMPERIAL/2008/New/Wet_Lab | Wet Lab Hub]] (explanation of contents of branches) [i] Tom - editor of all wetlab pages
***[[IGEM:IMPERIAL/2008/New/Protocols | Protocols]] [J]
***[[IGEM:IMPERIAL/2008/New/Major_Results | Major Results]] [w]
***[[IGEM:IMPERIAL/2008/New/Cloning_Strategy | Cloning Strategy]] [C]
***[[IGEM:IMPERIAL/2008/New/Biobricks | Biobricks and Characterisation]] [w]
**[[IGEM:IMPERIAL/2008/New/Dry_Lab | Dry Lab Hub]] [i] Erika - editor of all drylab pages
***[[IGEM:IMPERIAL/2008/New/Growth_Curve | Growth Curve]] [E, P]
***[[IGEM:IMPERIAL/2008/New/Genetic_Circuit | Genetic Circuit]] [E]
***[[IGEM:IMPERIAL/2008/New/Motility | Motility]] [L, Y]
***[[IGEM:IMPERIAL/2008/New/Appendices | Appendices]] (Code, protocols, programs used) [d]
 
 
Basically please note which pages you are responsible for. When we get to updating the wiki (to put it on the official one) we'll each need to summarise our pages and put them up, ''fixing any links and re-uploading any images/files'' so they point to the right place! If you have any problems or are unsure of how to do something, ask Tom or QQ. By the end of week 10, please could everyone have a relevant summary of their work - along with ''necessary'' diagrams and results - up on these pages so we can see what we've got.
 
Keep working and putting things on the "Prototype" wiki, and use this for finished work and so on. Try not to make new pages - we're aiming for a small wiki, concisely written but with all the necessary information. Remember to ask us (or bring it up at a debrief!) if you're having trouble, think it could be organised better a different way, or anything else.
 
~Tom and QQ
 
{{Imperial/EndPage}}

Latest revision as of 09:14, 13 October 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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Welcome to the Imperial 2008 iGEM project page. It's Wednesday, April 24 and a great day to read about an awesome iGEM project!


<html><center><img width="350px" src="http://i59.photobucket.com/albums/g305/Timpski/Logo1.png"></center></html>

For the 2008 iGEM competition, the Imperial College Team aims to develop a genetically-engineered Biofabricator, using the Gram-positive bacterium Bacillus subtilis as our chassis. Our Biofabricator aims to produce self-assembling biomaterials in specified 3D shapes, using light as the trigger.

This is achieved in three stages. First by utilising an endogenous light-sensing mechanism, the bacteria is captured in the desired location using 3D holography. Next bacterial locomotion is suspended in the region of interest using a recently-discovered clutch mechanism. This involves disengaging the flagellum from the motor protein. Finally, when our bacteria are stationary in the correct location, the biomaterial production is triggered. These biomaterials can self-assemble to form a 3D bio-scaffold. Applications of our Biofabricator range from regenerative tissue engineering to Bio-Couture.

Overview of our planned system
Overview of our planned system
Basic Circuit Diagram
Basic Circuit Diagram



Please continue on to our project pages - you may want to start with our >>> Project Specifications >>>


The Imperial College Team 2008 has received sponsorship from a number of generous companies. We are grateful for their kind support. <html><center><a href="http://www.bio-rad.com/"><img height="40px" src="http://i59.photobucket.com/albums/g305/Timpski/Biorad.png"></a><a href="http://www.fisher.co.uk/"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/Fisher.png"></a><a href="http://www.geneart.com/"><img height="25px" src="http://i59.photobucket.com/albums/g305/Timpski/Geneart.png"></a><a href="http://www.vwr.com/index.htm"><img height="50px" src="http://i59.photobucket.com/albums/g305/Timpski/VWR.png"></a></center></html>



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