IGEM:IMPERIAL/2008/New/Home: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 12: | Line 12: | ||
For the 2008 iGEM competition, the Imperial | For the 2008 iGEM competition, the Imperial team is working on the foundations for a biofabricator. We are using the Gram-positive ''Bacillus subtilis'' bacterium as our chassis and hope to exert fine control over its movement via a recently-discovered clutch mechanism. Using light as our stimulus to localise the bacteria, we then intend to trigger production and secretion of a self-assembling bio-scaffold material in a set 3D pattern. | ||
The following diagram gives an overview of how our system works. Initially, ''B. subtilis'' are motile and are not producing biomaterials. If we want to construct a bio-scaffold with a 3D "I" shape, we shine a 3D hologram of the correct wavelength (red is used as an arbitrary example here) onto the growth medium. | |||
Line 21: | Line 21: | ||
| [[Image:Imperial_2008_Bioprinter_Cartoon.png |400px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]] | | [[Image:Imperial_2008_Bioprinter_Cartoon.png |400px| Overview of our planned system]] [[Image:Imperial_2008_Basic_Circuit.jpg | Basic Circuit Diagram]] | ||
| valign="top" | | | valign="top" | | ||
<br>Bacteria will sense that light | <br>Bacteria will sense that light and triggers start to produce a clutch molecule. This disengages the flagella from the motor quite quickly, rendering the ''subtilis'' stationary. <br><br>Coupled with the clutch is a gene for expression for biomaterial synthesis. Should any individuals stray from the correct area, the clutch should disengage and material synthesis should stop. | ||
<br><br>3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabrication process. | <br><br>3D bio-scaffold materials have many applications in tissue engineering and regenerative medicine. We hope to build up our bio-scaffold material pixel by pixel in the defined area - the basis of our 3D biofabrication process. | ||
|} | |} |
Revision as of 06:49, 11 September 2008
<html> <style type="text/css"> .firstHeading {display: none;} </style> </html> <html> <style type="text/css">
table.calendar { margin:0; padding:2px; }
table.calendar td { margin:0; padding:1px; vertical-align:top; } table.month .heading td { padding:1px; background-color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td { text-align:center; font-size:110%; } table.month td.today { background-color:#3366FF } table.month td {
border:2px; margin:0; padding:0pt 1.5pt; font-size:8pt; text-align:right; background-color:#FFFFFF; }
- bodyContent table.month a { background:none; padding:0 }
.day-active { font-weight:bold; } .day-empty { color:black; } </style> </html>