IGEM:IMPERIAL/2008/New/Growth Curve
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  {{Imperial/  +  {{Imperial/Box2Growth Curve 
Part of our dry lab team concentrated on modelling the growth curve of ''B. subtilis''. This is important to characterise the chassis; particularly, the growth of ''subtilis'' is a vital parameter for planning experiments for future projects. Characterisation increases the predictability of the growth of B. subtilis by determining, for example, its growth rate and the duration of its distinctive growth phases. In order to model the growth of ''B. subtilis'', the process was broken down into three main steps, where a separate submodel is produced in MATLAB for each step. Each submodel is an ODE model, which can be simulated using MATLAB. The variables in each submodel can be adjusted according to the boundary conditions (from experimental results).  Part of our dry lab team concentrated on modelling the growth curve of ''B. subtilis''. This is important to characterise the chassis; particularly, the growth of ''subtilis'' is a vital parameter for planning experiments for future projects. Characterisation increases the predictability of the growth of B. subtilis by determining, for example, its growth rate and the duration of its distinctive growth phases. In order to model the growth of ''B. subtilis'', the process was broken down into three main steps, where a separate submodel is produced in MATLAB for each step. Each submodel is an ODE model, which can be simulated using MATLAB. The variables in each submodel can be adjusted according to the boundary conditions (from experimental results).  
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[[Image:Log_graph.tif300pxLogGraph used to determine the growth rate]]}}  [[Image:Log_graph.tif300pxLogGraph used to determine the growth rate]]}}  
  {{Imperial/  +  {{Imperial/Box1Key Phases of Bacterial Growth 
===Lag Phase===  ===Lag Phase===  
During the lag phase, the rate of growth is slow due to two main reasons, ''B. subtilis'' is absorbing nutrients in the medium and the replication machinery is being switched on. The higher the concentration of nutrients in the medium, the faster the rate of bateria growth.  During the lag phase, the rate of growth is slow due to two main reasons, ''B. subtilis'' is absorbing nutrients in the medium and the replication machinery is being switched on. The higher the concentration of nutrients in the medium, the faster the rate of bateria growth. 
Revision as of 06:48, 30 September 2008
 
