A simple synthesisdegradation model is assumed for the modelling of the expression of a protein under the control of a constitutive promoter, with the same model assumed for all four promoterRBS constructs. The synthesisdegradation model assumes a steady state level of mRNA.
In this case, [protein] represents the concentration of GFP, k_{1} represents the rate of sythesis and d_{1} represents the degradation rate.
We can easily simulate this synthesisdegradation model using matlab:
ODE
Simulation File
We can also solve this ODE analytically.
Consider the steadystate behaviour of [protein].
This relationship can be seen in the parameter scan graphs on the right.
From the wetlab experiments it is likely that we will obtain steadystate data for each of the four promoterRBS constructs. If we assume the same rate of degradation of GFP in each case, we can have some measure of the relative rate of transcription through each promoter which will help us with the selection of the most appropriate promoter to use for Phase 2. In order to obtain an absolute measure of transcription (as opposed to a relative measure of transcriptional strength) we require constitutive expression in terms of molecules per cell (as opposed to fluorescene in arbitrary units).
Note from the parameter scan graphs:
 In the case where k_{1} = 0, no GFP is sythesised.
 In the case where d_{1} = 0, the concentration of protein does not reach a steady state.

Constitutive expression of antibiotic resistance (AB) and GFP. GFP brick is part E0040, GFPmut3b. Terminator is part B0015, the doublestop.
