IGEM:IMPERIAL/2008/New/Genetic Circuit: Difference between revisions
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[[Media:SimulationGfp.m|Simulation File]] | [[Media:SimulationGfp.m|Simulation File]] | ||
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We can also solve this ODE analytically. Consider the steady-state behaviour of <math>[protein]</math>. | We can also solve this ODE analytically. Consider the steady-state behaviour of <math>[protein]</math>. | ||
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This relationship can be seen in the parameter scan graphs. | This relationship can be seen in the parameter scan graphs. | ||
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Image:ParamterScanGFPk1.jpg|Parameter scan on <math>k_1</math> | |||
Image:ParamterScanGFPd1.jpg|Parameter scan on <math>d_1</math> | |||
</gallery> | |||
From the wetlab experiments it is likely that we will obtain steady-state data for each of the four promoter-RBS constructs. If we assume the same rate of degradation of GFP in each case, we can have some measure of the relative rate of transcription through each promoter which will help us with the selection of the most appropriate promoter to use for Phase 2. In order to obtain an absolute measure of transcription (as opposed to a relative measure of transcriptional strength) we require constitutive expression in terms of molecules per cell (as opposed to fluorescene in arbitrary units). | From the wetlab experiments it is likely that we will obtain steady-state data for each of the four promoter-RBS constructs. If we assume the same rate of degradation of GFP in each case, we can have some measure of the relative rate of transcription through each promoter which will help us with the selection of the most appropriate promoter to use for Phase 2. In order to obtain an absolute measure of transcription (as opposed to a relative measure of transcriptional strength) we require constitutive expression in terms of molecules per cell (as opposed to fluorescene in arbitrary units). | ||
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Revision as of 04:43, 29 September 2008
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