IGEM:IMPERIAL/2008/New/Cloning Strategy - Revision history

Qin Qi at 10:25, 6 October 2008

Qin Qi — Mon, 06 Oct 2008 10:25:03 GMT

←Older revision		Revision as of 10:25, 6 October 2008
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	{{Imperial/Box1\|\|		{{Imperial/Box1\|\|
-	~~Our~~ cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.	+	The cloning strategy for our Biofabricator is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
	<br><br>		<br><br>
	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]		[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]

Tom Adie at 12:16, 30 September 2008

Tom Adie — Tue, 30 Sep 2008 12:16:19 GMT

←Older revision		Revision as of 12:16, 30 September 2008
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	{{Imperial/StartPage2}}__NOTOC__		{{Imperial/StartPage2}}__NOTOC__
-	~~<br>~~
-	~~<br>~~
-	~~  <font size=6px color=#E5EBFF><b>Cloning Strategy</b></font>~~
-	~~<br>~~
-	~~<br>~~

-	{{Imperial/Box1\|\|The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up presents many challenges.

		+	{{Imperial/Box2\|Cloning Strategy\|
		+	The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up also presents many challenges.}}
		+
		+	{{Imperial/Box1\|\|
	Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.		Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
	<br><br>		<br><br>
	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]		[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]
-	<br><br>	+	<br><br>}}
		+
		+	{{Imperial/Box1\|Constructs\|
	A summary of the aims of each phase and constructs to be produced within each phase, is given below:		A summary of the aims of each phase and constructs to be produced within each phase, is given below:

Qin Qi at 17:22, 27 September 2008

Qin Qi — Sat, 27 Sep 2008 17:22:42 GMT

←Older revision		Revision as of 17:22, 27 September 2008
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	<br><br>		<br><br>
	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]		[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]
-		+	<br><br>
-	<hr>	+
	A summary of the aims of each phase and constructs to be produced within each phase, is given below:		A summary of the aims of each phase and constructs to be produced within each phase, is given below:

Qin Qi at 17:22, 27 September 2008

Qin Qi — Sat, 27 Sep 2008 17:22:22 GMT

←Older revision		Revision as of 17:22, 27 September 2008
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	Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.		Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
	<br><br>		<br><br>
-	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]}}	+	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]
-		+
-	~~{{Imperial/Box1\|\|~~	+

		+	<hr>
	A summary of the aims of each phase and constructs to be produced within each phase, is given below:		A summary of the aims of each phase and constructs to be produced within each phase, is given below:

Qin Qi at 17:21, 27 September 2008

Qin Qi — Sat, 27 Sep 2008 17:21:50 GMT

←Older revision		Revision as of 17:21, 27 September 2008
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	<br><br>		<br><br>
	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]}}		[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]}}
		+
		+	{{Imperial/Box1\|\|

	A summary of the aims of each phase and constructs to be produced within each phase, is given below:		A summary of the aims of each phase and constructs to be produced within each phase, is given below:
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	Combination of light sensing and light-induced expression of epsE and biomaterial. Each gene has its own promoter/RBS pair because in ''B. subtilis'', it has been shown that levels of expression decrease as one moves along an operon.		Combination of light sensing and light-induced expression of epsE and biomaterial. Each gene has its own promoter/RBS pair because in ''B. subtilis'', it has been shown that levels of expression decrease as one moves along an operon.
	<p><html><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></html></p>		<p><html><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></html></p>
-		+	}}
	{{Imperial/EndPage\|Wet_Lab\|Protocols}}		{{Imperial/EndPage\|Wet_Lab\|Protocols}}

Qin Qi at 17:21, 27 September 2008

Qin Qi — Sat, 27 Sep 2008 17:21:03 GMT

←Older revision		Revision as of 17:21, 27 September 2008
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	Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.		Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
-		+	<br><br>
	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]}}		[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]}}

Qin Qi at 17:20, 27 September 2008

Qin Qi — Sat, 27 Sep 2008 17:20:43 GMT

←Older revision		Revision as of 17:20, 27 September 2008
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	Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.		Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.

-	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]	+	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]}}

-	A summary of the aims of each phase and constructs to be produced within each phase, is given below:}}	+	A summary of the aims of each phase and constructs to be produced within each phase, is given below:

	====== Phase 1: Testing of Constitutive Promoters ======		====== Phase 1: Testing of Constitutive Promoters ======

Qin Qi at 17:20, 27 September 2008

Qin Qi — Sat, 27 Sep 2008 17:20:19 GMT

←Older revision		Revision as of 17:20, 27 September 2008
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	{{Imperial/StartPage2}}__NOTOC__		{{Imperial/StartPage2}}__NOTOC__
-	= Cloning Strategy =	+	<br>
		+	<br>
		+	<font size=6px color=#E5EBFF><b>Cloning Strategy</b></font>
		+	<br>
		+	<br>

-	The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up presents many challenges.	+	{{Imperial/Box1\|\|The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up presents many challenges.

	Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.		Our cloning strategy is complex. In order to build the required constructs for our final product, we need to build, test and characterise intermediary parts and devices that will lead to the final system. The diagram below shows the critical pathway for our cloning strategy with a large number of closely-linked steps.
-

	[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]		[[Image:Imperial_2008_Critical_Pathway.png\|center\|700px]]

-		+	A summary of the aims of each phase and constructs to be produced within each phase, is given below:}}
-	A summary of the aims of each phase and constructs to be produced within each phase, is given below:	+

	====== Phase 1: Testing of Constitutive Promoters ======		====== Phase 1: Testing of Constitutive Promoters ======

Tom Adie at 17:01, 17 September 2008

Tom Adie — Wed, 17 Sep 2008 17:01:18 GMT

←Older revision		Revision as of 17:01, 17 September 2008
Line 38:		Line 38:
	Combination of light sensing and light-induced expression of epsE and biomaterial. Each gene has its own promoter/RBS pair because in ''B. subtilis'', it has been shown that levels of expression decrease as one moves along an operon.		Combination of light sensing and light-induced expression of epsE and biomaterial. Each gene has its own promoter/RBS pair because in ''B. subtilis'', it has been shown that levels of expression decrease as one moves along an operon.
	<p><html><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></html></p>		<p><html><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png"></html></p>
		+
		+	{{Imperial/EndPage\|Wet_Lab\|Protocols}}

Tom Adie at 19:43, 16 September 2008

Tom Adie — Tue, 16 Sep 2008 19:43:52 GMT

←Older revision		Revision as of 19:43, 16 September 2008
Line 1:		Line 1:
	{{Imperial/StartPage2}}__NOTOC__		{{Imperial/StartPage2}}__NOTOC__
-	= ~~'''~~Cloning Strategy~~'''~~ =	+	= Cloning Strategy =

	The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up presents many challenges.		The Imperial iGEM 2008 team faces the task of working with a chassis that has been rarely used - and never characterised - in the competition to date. While the ''B. subtilis'' chassis offers us many advantages, working from the ground up presents many challenges.
Line 12:		Line 12:
	A summary of the aims of each phase and constructs to be produced within each phase, is given below:		A summary of the aims of each phase and constructs to be produced within each phase, is given below:

-	====== Phase 1: Testing of Constitutive ~~Promoter~~ ======	+	====== Phase 1: Testing of Constitutive Promoters ======
	We will test 4 combinations of 2 constitutive promoters and 2 RBSs to characterise them. An antibiotic resistance cassette is placed at the 5' end of the construct, to prevent any readthrough by the native trancriptases from reaching the regulated BioBricks.		We will test 4 combinations of 2 constitutive promoters and 2 RBSs to characterise them. An antibiotic resistance cassette is placed at the 5' end of the construct, to prevent any readthrough by the native trancriptases from reaching the regulated BioBricks.