IGEM:IMPERIAL/2008/New/Cloning Strategy

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(Cloning Strategy)
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= Cloning Strategy =
= Cloning Strategy =
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The Imperial iGEM 2008 team is facing a mammoth build in an organism that is relatively unused in the competition. This leaves us with many challenges to overcome, in particular having to work from the ground up.
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The Imperial iGEM 2008 team is facing a mammoth task of working on a chassis that has been rarely used in the competition so far. This leaves us with many challenges to overcome, in particular having to work from the ground up.  
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This complicates yet also guides our stratergy, as in order to make increasingly complicated constructs we must first build and test simpler devices from which to take components to build our finished product
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This also makes our cloning stratergy highly complicated. In order to building increasingly complicated constructs for our final product, we must first build and test simpler parts and devices.
[[Image:Imperial_2008_Critical_Pathway.png|center|800px|]]
[[Image:Imperial_2008_Critical_Pathway.png|center|800px|]]

Revision as of 06:06, 8 September 2008

Home The Project B.subtilis Chassis Wet Lab Dry Lab Notebook


Authors: Chris

Editors: Wetlab team, wiki team

Cloning Strategy

The Imperial iGEM 2008 team is facing a mammoth task of working on a chassis that has been rarely used in the competition so far. This leaves us with many challenges to overcome, in particular having to work from the ground up.

This also makes our cloning stratergy highly complicated. In order to building increasingly complicated constructs for our final product, we must first build and test simpler parts and devices.

This is the overall cloning sratergy for our project. A summary of the constructs that should be produced during each phase and will be used to test parts and devices is shown below:

Phase 1
Phase 2A Phase 2B
Phase 3A Phase 3B
Phase 4A Phase 4B
Final Construct

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