IGEM:IMPERIAL/2008/New/Chassis 2

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<html><a href=http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype><img width=50px src=http://openwetware.org/images/f/f2/Imperial_2008_Logo.png></img</a></html> Home The Project B.subtilis Chassis Wet Lab Dry Lab Notebook

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Chassis 2 - Other pros, cons and information...

http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Project

Authors: Chris, James

Editors: Wiki team

James - Here is a table of potential advantages and disadvatages of using B.subtilis for our project and some more general chassis ad/disad. We may wish to think about chassis ad/dis seperatly, for example, in the registry the we will want to put the chassis info up!

Benefits
Challenges
Single Membrane - B.subtilis is a gram-positive bacterium and contains only a single membrane. This makes B.subtilis an ideal organism for secretion of proteins and other biomaterials. Starting from scratch - Although previous iGEM teams have looked at B.subtilis as a potential chassis, there are few working B.subtilis parts. As a result we will have to design a vaerity of promoter, ribosome binding sites and protein coding regions.
Potential Parts - B.subtilis is a highly studied organism, its genome has been fully sequenced and many of these genes have been functionaly annotated. As a result, B.subtilis provides many useful potential parts that we could take advantage of. For example within our project, potential parts for control of motility, light sensing and secretion pathways. Stationary Phase and sporulation
Non pathogenic - Peptidase activity -
Naturally Competent Vector digestor -
Easy to store and transport (sporulation)

Highly Motile -




The Ugly

  • Take a sniff.... go on, just a quick one!



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