IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
mNo edit summary |
|||
Line 25: | Line 25: | ||
|style="background:#99BBFF" width=50%| | |style="background:#99BBFF" width=50%| | ||
|- | |- | ||
|style="background:#FFFFFF"| '''Availability of Potential Parts''' - ''B.subtilis'' is a highly studied organism. Its genome has been fully sequenced and many of these genes have been functionally annotated. As a result, ''B.subtilis'' provides many useful potential parts that we could take advantage of. For example within our project, potential parts for control of motility, light sensing and secretion pathways. | | rowspan=2 style="background:#FFFFFF"| '''Availability of Potential Parts''' - ''B.subtilis'' is a highly studied organism. Its genome has been fully sequenced and many of these genes have been functionally annotated. As a result, ''B.subtilis'' provides many useful potential parts that we could take advantage of. For example within our project, potential parts for control of motility, light sensing and secretion pathways. | ||
|style="background:#FFFFFF"| '''Biobricking''' - Although there is a huge number of potential parts, these still have to be manipulated to conform to existing Biobrick standards. | |style="background:#FFFFFF"| '''Biobricking''' - Although there is a huge number of potential parts, these still have to be manipulated to conform to existing Biobrick standards. | ||
|- | |- | ||
|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B.subtilis'' as a potential chassis, there are very few working ''B.subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | |style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B.subtilis'' as a potential chassis, there are very few working ''B.subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | ||
|- | |- | ||
Line 41: | Line 40: | ||
|- | |- | ||
|style="background:#FFFFFF"| '''Non-pathogenicity''' - ''B. subtilis'' is a non-pathogenic organism that is commonly found in soil. As a result, ''B. subtilis'' has a biological harzardous level of 1 and offers a useful non-pathogenic chassis for synthetic biologists. | |style="background:#FFFFFF"| '''Non-pathogenicity''' - ''B. subtilis'' is a non-pathogenic organism that is commonly found in soil. As a result, ''B. subtilis'' has a biological harzardous level of 1 and offers a useful non-pathogenic chassis for synthetic biologists. | ||
|style="background:#EEEEEE" width=50%| | | rowspan=3 style="background:#EEEEEE" width=50%| | ||
|- | |- | ||
|style="background:#FFFFFF"|'''High Motility''' - ''B.subtilis'' is often referred to as a highly motile organsim. Quoted velocity range from 60 - 100 μm per second. In comparison to ''E.coli'' which has an average velocity of .... ''B.subtilis'' is much more motile. | |style="background:#FFFFFF"|'''High Motility''' - ''B.subtilis'' is often referred to as a highly motile organsim. Quoted velocity range from 60 - 100 μm per second. In comparison to ''E.coli'' which has an average velocity of .... ''B.subtilis'' is much more motile. | ||
|- | |- | ||
|style="background:#FFFFFF"|'''Sporulation: Transport''' - Under stress conditions, ''B.subtilis'' will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting ''B.subtilis'' devices and constructs. | |style="background:#FFFFFF"|'''Sporulation: Transport''' - Under stress conditions, ''B.subtilis'' will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting ''B.subtilis'' devices and constructs. | ||
|} | |} | ||
<br> | <br> |
Revision as of 14:51, 9 September 2008
<html> <style type="text/css"> .firstHeading {display: none;} </style> </html> <html> <style type="text/css">
table.calendar { margin:0; padding:2px; }
table.calendar td { margin:0; padding:1px; vertical-align:top; } table.month .heading td { padding:1px; background-color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td { text-align:center; font-size:110%; } table.month td.today { background-color:#3366FF } table.month td {
border:2px; margin:0; padding:0pt 1.5pt; font-size:8pt; text-align:right; background-color:#FFFFFF; }
- bodyContent table.month a { background:none; padding:0 }
.day-active { font-weight:bold; } .day-empty { color:black; } </style> </html>
<html><script language="JavaScript">
var timeout = 250; var closetimer = 0; var ddmenuitem = 0; // open hidden layer function mopen(id) { // cancel close timer mcancelclosetime(); // close old layer if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; // get new layer and show it ddmenuitem = document.getElementById(id); ddmenuitem.style.visibility = 'visible'; } // close showed layer function mclose() { if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; } // go close timer function mclosetime() { closetimer = window.setTimeout(mclose, timeout); } // cancel close timer function mcancelclosetime() { if(closetimer) { window.clearTimeout(closetimer); closetimer = null; } } // close layer when click-out //document.onclick = mclose; </script> <table background="http://i59.photobucket.com/albums/g305/Timpski/ToolbarBackground.png" style="color:#ffffff;" link="#ffffff" cellpadding="0" cellspacing="1" border="0" bordercolor="#ffffff" align="center" width="100%"><tr><td colspan="6" class="wetlab"><br><br><br></td></tr> <tr><td align="center" width="10%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Home"> Home </a></ul> </td><td align="center" width="20%" valign="bottom"><ul id="sddm"><a href="#" onclick="mopen('m1')" onmouseover="mopen('m1')" onmouseout="mclosetime()">Biofabricator Subtilis</a> <div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a> </div></ul> </td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#" onclick="mopen('m2')" onmouseover="mopen('m2')" onmouseout="mclosetime()">Wet Lab</a> <div id="m2" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Wet_Lab">Wet Lab Hub</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Cloning_Strategy">Cloning Strategy</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a> </div></ul> </td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#" onclick="mopen('m3')" onmouseover="mopen('m3')" onmouseout="mclosetime()">Dry Lab</a> <div id="m3" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Dry_Lab">Dry Lab Hub</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Growth_Curve">Growth Curves</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Genetic_Circuit">Genetic Circuits</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a> <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a> </div></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html> | |||||||||||||||||||||
<html><style type="text/css">
div.Section { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; background-image: url(http://openwetware.org/images/a/a0/Background.PNG); background-size: 100%; background-origin: content; } /* Text (paragraphs) */ div.Section p { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; margin-top:0px; margin-left:30px; margin-right:30px; } /* Headings */ div.Section h1 { font:22pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; color:#3366FF; font-weight:bold; } /* Subheadings */ div.Section h2 { font:18pt Calibri, Verdana, Arial, Geneva, sans-serif; color:#3366FF; margin-left:5px; font-weight:bold; } /* Subsubheadings */ div.Section h3 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#E5EBFF; margin-left:10px; font-weight:bold; } /* Subsubsubheadings */ div.Section h4 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#2B48B3; margin-left:10px; font-weight:bold; } /* Subsubsubsubheadings */ div.Section h5 { font:12pt Calibri, Verdana, Arial, sans-serif; color:#3366FF; margin-left:20px; } /* References */ div.Section h6 { font:12pt Calibri, Verdana, Arial, sans-serif; font-weight:bold; font-style:italic; color:#3366FF; margin-left:25px; } /* Hyperlinks */ div.Section a { } div.Section a:hover { } /* Tables */ div.Section td { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; vertical-align:top; padding:2px 4px 2px 4px; } /* Lists */ div.Section li { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; margin-top:0px; margin-left:30px; margin-right:0px; } /* TOC stuff */ table.toc { margin-left:10px; } table.toc li { font: 11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align: justify; margin-top: 0px; margin-left:2px; margin-right:2px; } /* [edit] links */ span.editsection { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; } span.editsection a { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; } span.editsection a:hover { color:#3366FF; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; } /* Drop-down Menu */
margin: 0; padding: 0; z-index: 30 margin: 0; padding: 0; float: center; font: bold 12pt Calibri, Verdana, Arial, Geneva, sans-serif; border: 0px; list-style: none; }
display: block; margin: 0px 0px 0px 0px; padding: 0 0 12px 0; color: #FFFFFF; text-align: center; text-decoration: none; }
border: 0px }
position: absolute; visibility: hidden; margin: 0; padding: 0; background: #66aadd; border: 1px solid #66aadd } #sddm div a { position: relative; left: 0; display: block; margin: 0; padding: 5px 10px; width: auto; white-space: nowrap; text-align: left; text-decoration: none; background: #FFFFFF; color: #2875DE; font: 11pt Calibri, Verdana, Arial, Geneva, sans-serif } #sddm div a:hover { background: #66aadd; color: #FFFFFF } </style></html> Benefits vs Challengeshttp://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Project Authors: Chris, James Editors: Wiki team James - Here is a table of potential advantages and disadvatages of using B.subtilis for our project and some more general chassis ad/disad. We may wish to think about chassis ad/dis seperatly, for example, in the registry the we will want to put the chassis info up!
|