IGEM:IMPERIAL/2008/New/Chassis 2

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Line 9: Line 9:
!width="50%"|<font size=4px><font color=white><center>'''Challenges'''</center>
!width="50%"|<font size=4px><font color=white><center>'''Challenges'''</center>
|-
|-
-
|width="15" style="background:#99BBFF"|
+
|style="background:#99BBFF" colspan="3"|<font size=3px><font color=white><center>'''Protein Expression'''</center>
-
|style="background:#99BBFF"|<font size=3px><font color=white><center>'''Protein Expression'''</center>
+
-
|width="200" style="background:#99BBFF"|
+
|-
|-
|style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]]  
|style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]]  
Line 17: Line 15:
|style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted.
|style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted.
|-
|-
-
|style="background:#99BBFF"|
+
|style="background:#99BBFF" colspan="3"|<font size=3px><font color=white><center>'''Foundational Technology'''</center>
-
|style="background:#99BBFF"|<font size=3px><font color=white><center>'''Foundational Technology'''</center>
+
-
|style="background:#99BBFF"|
+
|-
|-
|rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]]
|rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]]
Line 27: Line 23:
|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B. subtilis'' as a potential chassis, there are very few working ''B. subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions.
|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B. subtilis'' as a potential chassis, there are very few working ''B. subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions.
|-
|-
-
|style="background:#99BBFF" |
+
|style="background:#99BBFF" colspan="3"|<font size=3px><font color=white><center>'''BioBrick Assembly</center>
-
|style="background:#99BBFF" |<font size=3px><font color=white><center>'''BioBrick Assembly</center>
+
-
|style="background:#99BBFF"|
+
|-
|-
|style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]]
|style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]]
Line 35: Line 29:
|style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E. coli''. One reason for this is that ''B. subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information.
|style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E. coli''. One reason for this is that ''B. subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information.
|-
|-
-
|style="background:#99BBFF"|
+
|style="background:#99BBFF" colspan="3"|<font size=3px><font color=white><center>'''Chassis Properties'''</center>
-
|style="background:#99BBFF"|<font size=3px><font color=white><center>'''Chassis Properties'''</center>
+
-
|style="background:#99BBFF"|
+
|-
|-
| rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]]
| rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]]

Revision as of 17:01, 16 September 2008




Benefits vs Challenges

Benefits
Challenges
Protein Expression
Single Membrane - B. subtilis is a gram-positive bacterium and contains only a single membrane. This makes B. subtilis an ideal chassis for secretion of biomaterials. Peptidase activity - B. subtilis express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted.
Foundational Technology
Availability of Potential Parts - B. subtilis is a highly studied organism, with a fully sequenced genome. As a result, B. subtilis provides many useful potential parts. BioBricking - Although there is a huge number of potential parts, these still have to be manipulated to conform to existing BioBrick standards.
Starting from scratch - Although previous iGEM teams have looked at B. subtilis as a potential chassis, there are very few working B. subtilis parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions.
BioBrick Assembly
Natural Competency and Integration - B. subtilis has been noted for its ease and efficieny of transformation. In addition, integration of exogenous DNA into the chromosome has been well studied and provides an alternative to using traditional plasmids. Vector Degradation - B. subtilis does not use all the same vectors as E. coli. One reason for this is that B. subtilis often recognises vectors grown in E. coli as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information.
Chassis Properties
Non-pathogenicity - B. subtilis is a non-pathogenic organism that is commonly found in soil. As a result, B. subtilis has a biological harzardous level of 1 and offers a useful non-pathogenic chassis for synthetic biologists.
High Motility - B.subtilis is often referred to as a highly motile organism in comparison to other bacterium.
Sporulation: Transport - Under stress conditions, B. subtilis will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting B. subtilis devices and constructs.



Having decided what we're doing and how we plan to do it, the next step is to get started! To see how our lab work went please start with the >>> Wet Lab Hub >>>

Retrieved from "http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2"
Personal tools