IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
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<font size=4px><font color=white><center>'''Benefits'''</center> | |||
!width="200"|<font size=4px><font color=white><center>'''Challenges'''</center> | |||
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|style="background:#99BBFF" | |width="200" style="background:#99BBFF"|<font size=3px><font color=white><center>'''Protein Expression'''</center> | ||
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|style="background:#FFFFFF"| '''Single Membrane''' - ''B.subtilis'' is a gram-positive bacterium and contains only a single membrane. This makes ''B.subtilis'' an ideal | |style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]] | ||
|style="background:#FFFFFF"| | |style="background:#FFFFFF"| '''Single Membrane''' - ''B.subtilis'' is a gram-positive bacterium and contains only a single membrane. This makes ''B.subtilis'' an ideal chassis for secretion of biomaterials. | ||
|style="background:#FFFFFF"| '''Peptidase activity''' - ''B.subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | |||
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|style="background:#99BBFF" | |style="background:#99BBFF"|<font size=3px><font color=white><center>'''Foundational Technology'''</center> | ||
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| rowspan=2 style="background:#FFFFFF"| '''Availability of Potential Parts''' - ''B.subtilis'' is a highly studied organism | |rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | ||
| rowspan=2 style="background:#FFFFFF"| '''Availability of Potential Parts''' - ''B.subtilis'' is a highly studied organism, with a fully sequenced genome. As a result, ''B.subtilis'' provides many useful potential parts. | |||
|style="background:#FFFFFF"| '''Biobricking''' - Although there is a huge number of potential parts, these still have to be manipulated to conform to existing Biobrick standards. | |style="background:#FFFFFF"| '''Biobricking''' - Although there is a huge number of potential parts, these still have to be manipulated to conform to existing Biobrick standards. | ||
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|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B.subtilis'' as a potential chassis, there are very few working ''B.subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | |style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B.subtilis'' as a potential chassis, there are very few working ''B.subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | ||
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|style="background:#99BBFF" | |style="background:#99BBFF" |<font size=3px><font color=white><center>'''Biobrick Assembly</center> | ||
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|style="background:#FFFFFF"| '''Natural Competency and Integration''' - ''B. subtilis'' has been noted for its ease and efficieny of transformation | |style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]] | ||
|style="background:#FFFFFF"| '''Natural Competency and Integration''' - ''B. subtilis'' has been noted for its ease and efficieny of transformation. In addition, inegration of exogenous DNA into the chromosome has been wel studied and provides an alternative to using traditional plasmids. | |||
|style="background:#FFFFFF"| '''Vector Degradation''' - ''B.subtilis'' does not use all the same vectors as ''E.coli''. One reason for this is that ''B.subtilis'' often recognises vectors grown in ''E.coli'' and digests them. This will (judging by the approach of previous iGEM teams) include the biobrick itself. | |style="background:#FFFFFF"| '''Vector Degradation''' - ''B.subtilis'' does not use all the same vectors as ''E.coli''. One reason for this is that ''B.subtilis'' often recognises vectors grown in ''E.coli'' and digests them. This will (judging by the approach of previous iGEM teams) include the biobrick itself. | ||
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|style="background:#99BBFF" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" | |style="background:#99BBFF"|<font size=3px><font color=white><center>'''Chassis Properties'''</center> | ||
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| rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]] | |||
|style="background:#FFFFFF"| '''Non-pathogenicity''' - ''B. subtilis'' is a non-pathogenic organism that is commonly found in soil. As a result, ''B. subtilis'' has a biological harzardous level of 1 and offers a useful non-pathogenic chassis for synthetic biologists. | |style="background:#FFFFFF"| '''Non-pathogenicity''' - ''B. subtilis'' is a non-pathogenic organism that is commonly found in soil. As a result, ''B. subtilis'' has a biological harzardous level of 1 and offers a useful non-pathogenic chassis for synthetic biologists. | ||
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|style="background:#FFFFFF"|'''High Motility''' - ''B.subtilis'' is often referred to as a highly motile organsim | |style="background:#FFFFFF"|'''High Motility''' - ''B.subtilis'' is often referred to as a highly motile organsim in comparison to other bacterium. | ||
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|style="background:#FFFFFF"|'''Sporulation: Transport''' - Under stress conditions, ''B.subtilis'' will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting ''B.subtilis'' devices and constructs. | |style="background:#FFFFFF"|'''Sporulation: Transport''' - Under stress conditions, ''B.subtilis'' will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting ''B.subtilis'' devices and constructs. |
Revision as of 03:13, 11 September 2008
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