IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
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|style="background:#FFFFFF"|'''Peptidase activity''' - | |style="background:#FFFFFF"|'''Peptidase activity''' - | ||
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|style="background:#FFFFFF"| '''Naturally Competent''' - | |style="background:#FFFFFF"| '''Naturally Competent''' - ''B.subtilis'' has been noted for its ease and efficieny of transformatio. | ||
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|style="background:#FFFFFF"|'''Highly Motile''' - | |style="background:#FFFFFF"|'''Highly Motile''' - ''B.subtilis'' is often referred to as a highly motile organsim. Quoted velocity range from 60 - 100 μm per second. In comparison to ''E.coli'' which has an average velocity of .... ''B.subtilis'' is much more motile. | ||
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Revision as of 11:08, 5 September 2008
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| <html><a href=http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype><img width=50px src=http://openwetware.org/images/f/f2/Imperial_2008_Logo.png></img</a></html> | Home | The Project | B.subtilis Chassis | Wet Lab | Dry Lab | Notebook |
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Chassis 2 - Other pros, cons and information...
http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Project
Authors: Chris, James
Editors: Wiki team
James - Here is a table of potential advantages and disadvatages of using B.subtilis for our project and some more general chassis ad/disad. We may wish to think about chassis ad/dis seperatly, for example, in the registry the we will want to put the chassis info up!
| Single Membrane - B.subtilis is a gram-positive bacterium and contains only a single membrane. This makes B.subtilis an ideal organism for secretion of proteins and other biomaterials. | Starting from scratch - Although previous iGEM teams have looked at B.subtilis as a potential chassis, there are few working B.subtilis parts. As a result we will have to design a vaerity of promoter, ribosome binding sites and protein coding regions. |
| Potential Parts - B.subtilis is a highly studied organism, its genome has been fully sequenced and many of these genes have been functionaly annotated. As a result, B.subtilis provides many useful potential parts that we could take advantage of. For example within our project, potential parts for control of motility, light sensing and secretion pathways. | Sporulation - Transport - Under stress conditions B.subtilis will form spores. These spores are highly resistant versions of single cells, able to with stand extremes in heat and pH. These spores are capable of growing into a new organisms once favourable growing conditions are returned. Because of the resistance of spores, there is a great potential for manipulating them for transporting B.subtilis devices and constructs. |
| Non pathogenic - B.subtilis is a non-pathogenic organism that is commonly found in soil. As a result has a biological harzdous level of 1 and offers a useful non-pathogenic chassis for synthetic biologists. | Peptidase activity - |
| Naturally Competent - B.subtilis has been noted for its ease and efficieny of transformatio. | |
| Highly Motile - B.subtilis is often referred to as a highly motile organsim. Quoted velocity range from 60 - 100 μm per second. In comparison to E.coli which has an average velocity of .... B.subtilis is much more motile. |
The Ugly
- Take a sniff.... go on, just a quick one!
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