IGEM:IMPERIAL/2008/New/Chassis 1: Difference between revisions
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= Chassis 1 = | |||
This page offers a brief overview of how ''B. subtilis'' meets our main project specifications - to a much higher degree than ''E. coli''! You'll find information on the main mechanisms behind our proposed system, and some very interesting biology too. If you would like even ''more'' depth than the outlines below, you are welcome to visit our OpenWetWare pages on those topics - but don't get to engrossed and forget to come back! | |||
==Motility== | ==Motility== | ||
Revision as of 12:55, 3 September 2008
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| <html><a href=http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype><img width=50px src=http://openwetware.org/images/f/f2/Imperial_2008_Logo.png></img</a></html> | Home | The Project | B.subtilis Chassis | Wet Lab | Dry Lab | Notebook |
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Authors: Wiki team
Editors: Wiki team
Chassis 1
This page offers a brief overview of how B. subtilis meets our main project specifications - to a much higher degree than E. coli! You'll find information on the main mechanisms behind our proposed system, and some very interesting biology too. If you would like even more depth than the outlines below, you are welcome to visit our OpenWetWare pages on those topics - but don't get to engrossed and forget to come back!
Motility
http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Motility
Light Sensing
We have chosen light stimulus as our input for the Bioprinter. Light allows us to generate complex patterns with well defined edges, while gradients in wavelength and intensity will allow us to build up varying concentrations of biomaterial.
Photoreceptors which were considered in our design include rhodopsins, phytochromes, LOV and BLUF. Of these, B.Subtilis contains the blue light sensing protein YtvA which is related to the plant blue-light receptors phototropins. YtvA is a positive activator of the general stress transcription factor σB. Blue and not red light activates YtvA and thus induces σB activity. We could use σB which is expressed upon blue light illumination to activate the production of EpsE and hence control bacteria motility. However, there are other stressors which activate σB, hence we aim to make blue light the main activator of σB by controlling growth conditions.
Biomaterials
http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Biomaterials
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