IGEM:IMPERIAL/2008/New/BioBricks

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

<html><center><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png">| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>


Parts Submitted to the Registry

For now, here are the parts we will submit to the Registry. They can also be accessed here.

<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>


Production Status

This table shows our progress with each part:


Key
Submitted
Sequenced
Midiprepped
Fully verified
Partially Verified
Transformed
Ligation pending/Failed
Awaiting GeneArt
Cloning difficulties
Part Number Part Name Status
K143001 5' AmyE Integration Sequence Sequenced
K143002 3' AmyE Integration Sequence Sequenced
K143005 5' EpsE Integration Sequence Sequenced
K143006 3' EpsE Integration Sequence Sequenced
K143008 5' PyrD Integration Sequence Transformed
K143009 3' PyrD Integration Sequence Sequenced
K143031 Aad9 Spectinomycin Resistance Gene Sequenced
K143032 EpsE Molecular Clutch Gene of B. subtilis Sequenced
K143033 LacI (Lva-, N terminal deletion) Sequenced
K143034 LipA-EAK16 Fusion New Midiprep Sent for Sequencing
K143035 LipA-Human Elastin Fusion Sequenced
K143036 Xylose Operon Regulatory Protein Pending
K143037 Ytva Blue Light Receptor of B. subtilis Sequenced
K143038 SacB-EAK16 Fusion Sequenced
K143039 SacB-Human Elastin Fusion Sequenced
K143050 P43-gsiB Sequenced
K143051 P43-spoVG Sequenced
K143052 Pveg-gsiB Sequenced
K143053 Pveg-spoVG Sequenced
K143054 Phyperspank-gsiB Transformed
K143055 Phyperspank-spoVG Sequenced
K143056 Pxyl-gsiB Transformed
K143057 Pxyl-spoVG Prepared for Midiprep
K143058 Pctc-gsiB Sequenced
K143059 Pctc-spoVG Sequenced
K143060 PgsiB-gsiB Prepared for Midiprep
K143061 PgsiB-spoVG Sequenced
K143062 LacI - Terminator Sent for Sequencing
K143063 XylR - Terminator Pending
K143064 CAT - Terminator Sequenced
K143065 Aad9 - Terminator Sequenced
K143066 YtvA - Terminator Sequenced


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