IGEM:IMPERIAL/2008/New/BioBricks - Revision history

Chris D Hirst at 01:56, 29 October 2008

2008-10-29T01:56:53Z

←Older revision		Revision as of 01:56, 29 October 2008
Line 116:		Line 116:
	\|- style=background:gold		\|- style=background:gold
	\| K143072 \|\| AmyE integratable PoPS generator (P43-spoVG) (with CmR) \|\| Submitted		\| K143072 \|\| AmyE integratable PoPS generator (P43-spoVG) (with CmR) \|\| Submitted
-	\|- style=background:~~gold~~	+	\|- style=background:royalblue
-	\| K143073 \|\| AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) \|\| ~~Submitted~~	+	\| K143073 \|\| AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) \|\| Delayed
	\|- style=background:gold		\|- style=background:gold
	\| K143074 \|\| AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) \|\| Submitted		\| K143074 \|\| AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) \|\| Submitted

Chris D Hirst at 01:55, 29 October 2008

2008-10-29T01:55:57Z

←Older revision		Revision as of 01:55, 29 October 2008
Line 105:		Line 105:
	\| K143066 \|\| YtvA - Terminator \|\| Submitted		\| K143066 \|\| YtvA - Terminator \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143067 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143067 \|\| AmyE integratable PoPS generator (P43-gsiB) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143068 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143068 \|\| AmyE integratable PoPS generator (P43-spoVG) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143069 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143069 \|\| AmyE integratable PoPS generator (Pveg-gsiB) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143070 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143070 \|\| AmyE integratable PoPS generator (Pveg-spoVG) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143071 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143071 \|\| AmyE integratable PoPS generator (P43-gsiB) (with CmR) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143072 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143072 \|\| AmyE integratable PoPS generator (P43-spoVG) (with CmR) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143073 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143073 \|\| AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143074 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143074 \|\| AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143075 \|\| ~~YtvA - Terminator~~ \|\| Submitted	+	\| K143075 \|\| B. subtilis AmyE locus GFP output promoter and RBS tester \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143076 \|\| ~~YtvA - Terminator~~ \|\| Submitted	+	\| K143076 \|\| B. subtilis AmyE locus RFP output promoter and RBS tester \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143077 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143077 \|\| P43-gsiB GFP expression construct \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143078 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143078 \|\| P43-spoVG GFP expression construct \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143079 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143079 \|\| Pveg-spoVG GFP expression construct \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143080 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143080 \|\| P43-gsiB RFP expression construct \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143081 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143081 \|\| P43-spoVG RFP expression construct \|\| Submitted
	\|- style=background:gold		\|- style=background:gold
-	\| K143082 \|\| ~~YtvA~~ - ~~Terminator~~ \|\| Submitted	+	\| K143082 \|\| Pveg-spoVG RFP expression construct \|\| Submitted
	\|}		\|}

	{{Imperial/EndPage\|Major_Results\|Dry_Lab}}		{{Imperial/EndPage\|Major_Results\|Dry_Lab}}

Chris D Hirst at 01:52, 29 October 2008

2008-10-29T01:52:24Z

Show changes

Chris D Hirst at 17:45, 24 October 2008

2008-10-24T17:45:45Z

←Older revision		Revision as of 17:45, 24 October 2008
Line 48:		Line 48:
	\|- style=background:silver		\|- style=background:silver
	\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced		\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced
-	\|- style=background:~~yellow~~	+	\|- style=background:royalblue
-	\| K143008 \|\| 5' PyrD Integration Sequence \|\| ~~Pending~~	+	\| K143008 \|\| 5' PyrD Integration Sequence \|\| Transformed
	\|- style=background:silver		\|- style=background:silver
	\| K143009 \|\| 3' PyrD Integration Sequence \|\| Sequenced		\| K143009 \|\| 3' PyrD Integration Sequence \|\| Sequenced
Line 59:		Line 59:
	\| K143033 \|\| LacI (Lva<sup>-</sup>, N terminal deletion) \|\| Sequenced		\| K143033 \|\| LacI (Lva<sup>-</sup>, N terminal deletion) \|\| Sequenced
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143034 \|\| LipA-EAK16 Fusion \|\| ~~Partially Sequenced~~	+	\| K143034 \|\| LipA-EAK16 Fusion \|\| New Midiprep Sent for Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143035 \|\| LipA-Human Elastin Fusion \|\| Sequenced		\| K143035 \|\| LipA-Human Elastin Fusion \|\| Sequenced
-	\|- style=background:~~royalblue~~	+	\|- style=background:yellow
-	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| ~~Transformed~~	+	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Pending
	\|- style=background:silver		\|- style=background:silver
	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Sequenced		\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Sequenced
Line 78:		Line 78:
	\|- style=background:silver		\|- style=background:silver
	\| K143053 \|\| Pveg-spoVG \|\| Sequenced		\| K143053 \|\| Pveg-spoVG \|\| Sequenced
-	\|- style=background:~~yellow~~	+	\|- style=background:royalblue
-	\| K143054 \|\| Phyperspank-gsiB \|\| ~~Pending~~	+	\| K143054 \|\| Phyperspank-gsiB \|\| Transformed
	\|- style=background:silver		\|- style=background:silver
	\| K143055 \|\| Phyperspank-spoVG \|\| Sequenced		\| K143055 \|\| Phyperspank-spoVG \|\| Sequenced
-	\|- style=background:~~yellow~~	+	\|- style=background:royalblue
-	\| K143056 \|\| Pxyl-gsiB \|\| ~~Pending~~	+	\| K143056 \|\| Pxyl-gsiB \|\| Transformed
	\|- style=background:lawngreen		\|- style=background:lawngreen
	\| K143057 \|\| Pxyl-spoVG \|\| Prepared for Midiprep		\| K143057 \|\| Pxyl-spoVG \|\| Prepared for Midiprep
Line 94:		Line 94:
	\|- style=background:silver		\|- style=background:silver
	\| K143061 \|\| PgsiB-spoVG \|\| Sequenced		\| K143061 \|\| PgsiB-spoVG \|\| Sequenced
-	\|- style=background:~~royalblue~~	+	\|- style=background:#cc4400
-	\| K143062 \|\| LacI - Terminator \|\| ~~Transformed~~	+	\| K143062 \|\| LacI - Terminator \|\| Sent for Sequencing
	\|- style=background:yellow		\|- style=background:yellow
	\| K143063 \|\| XylR - Terminator \|\| Pending		\| K143063 \|\| XylR - Terminator \|\| Pending

Chris D Hirst at 21:05, 23 October 2008

2008-10-23T21:05:42Z

←Older revision		Revision as of 21:05, 23 October 2008
Line 44:		Line 44:
	\|- style=background:silver		\|- style=background:silver
	\| K143002 \|\| 3' AmyE Integration Sequence \|\| Sequenced		\| K143002 \|\| 3' AmyE Integration Sequence \|\| Sequenced
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143005 \|\| 5' EpsE Integration Sequence \|\| ~~Sent for Sequencing~~	+	\| K143005 \|\| 5' EpsE Integration Sequence \|\| Sequenced
	\|- style=background:silver		\|- style=background:silver
	\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced		\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced
Line 64:		Line 64:
	\|- style=background:royalblue		\|- style=background:royalblue
	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed		\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| ~~Sent for Sequencing~~	+	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Sequenced
	\|- style=background:silver		\|- style=background:silver
	\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced		\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced
Line 85:		Line 85:
	\| K143056 \|\| Pxyl-gsiB \|\| Pending		\| K143056 \|\| Pxyl-gsiB \|\| Pending
	\|- style=background:lawngreen		\|- style=background:lawngreen
-	\| K143057 \|\| Pxyl-spoVG \|\| ~~Sent~~ for ~~Partial Sequencing~~	+	\| K143057 \|\| Pxyl-spoVG \|\| Prepared for Midiprep
	\|- style=background:silver		\|- style=background:silver
	\| K143058 \|\| Pctc-gsiB \|\| Sequenced		\| K143058 \|\| Pctc-gsiB \|\| Sequenced
Line 91:		Line 91:
	\| K143059 \|\| Pctc-spoVG \|\| Sequenced		\| K143059 \|\| Pctc-spoVG \|\| Sequenced
	\|- style=background:lawngreen		\|- style=background:lawngreen
-	\| K143060 \|\| PgsiB-gsiB \|\| ~~Sent~~ for ~~Partial Sequencing~~	+	\| K143060 \|\| PgsiB-gsiB \|\| Prepared for Midiprep
	\|- style=background:silver		\|- style=background:silver
	\| K143061 \|\| PgsiB-spoVG \|\| Sequenced		\| K143061 \|\| PgsiB-spoVG \|\| Sequenced
Line 100:		Line 100:
	\|- style=background:silver		\|- style=background:silver
	\| K143064 \|\| CAT - Terminator \|\| Sequenced		\| K143064 \|\| CAT - Terminator \|\| Sequenced
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143065 \|\| Aad9 - Terminator \|\| ~~Sent for Sequencing~~	+	\| K143065 \|\| Aad9 - Terminator \|\| Sequenced
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143066 \|\| YtvA - Terminator \|\| ~~Sent for Sequencing~~	+	\| K143066 \|\| YtvA - Terminator \|\| Sequenced
	\|}		\|}

	{{Imperial/EndPage\|Major_Results\|Dry_Lab}}		{{Imperial/EndPage\|Major_Results\|Dry_Lab}}

Chris D Hirst at 14:45, 21 October 2008

2008-10-21T14:45:00Z

←Older revision		Revision as of 14:45, 21 October 2008
Line 45:		Line 45:
	\| K143002 \|\| 3' AmyE Integration Sequence \|\| Sequenced		\| K143002 \|\| 3' AmyE Integration Sequence \|\| Sequenced
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143005 \|\| 5' EpsE Integration Sequence \|\| ~~Midiprepped~~	+	\| K143005 \|\| 5' EpsE Integration Sequence \|\| Sent for Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced		\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced
Line 65:		Line 65:
	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed		\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| ~~Midiprepped~~	+	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Sent for Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced		\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143039 \|\| SacB-Human Elastin Fusion \|\| ~~Sent for Sequencing~~	+	\| K143039 \|\| SacB-Human Elastin Fusion \|\| Sequenced
	\|- style=background:silver		\|- style=background:silver
	\| K143050 \|\| P43-gsiB \|\| Sequenced		\| K143050 \|\| P43-gsiB \|\| Sequenced
Line 80:		Line 80:
	\|- style=background:yellow		\|- style=background:yellow
	\| K143054 \|\| Phyperspank-gsiB \|\| Pending		\| K143054 \|\| Phyperspank-gsiB \|\| Pending
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
	\| K143055 \|\| Phyperspank-spoVG \|\| Sequenced		\| K143055 \|\| Phyperspank-spoVG \|\| Sequenced
	\|- style=background:yellow		\|- style=background:yellow
Line 90:		Line 90:
	\|- style=background:silver		\|- style=background:silver
	\| K143059 \|\| Pctc-spoVG \|\| Sequenced		\| K143059 \|\| Pctc-spoVG \|\| Sequenced
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:lawngreen
-	\| K143060 \|\| PgsiB-gsiB \|\| ~~PCR Verified~~	+	\| K143060 \|\| PgsiB-gsiB \|\| Sent for Partial Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143061 \|\| PgsiB-spoVG \|\| Sequenced		\| K143061 \|\| PgsiB-spoVG \|\| Sequenced
Line 101:		Line 101:
	\| K143064 \|\| CAT - Terminator \|\| Sequenced		\| K143064 \|\| CAT - Terminator \|\| Sequenced
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143065 \|\| Aad9 - Terminator \|\| ~~Midiprepped~~	+	\| K143065 \|\| Aad9 - Terminator \|\| Sent for Sequencing
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143066 \|\| YtvA - Terminator \|\| ~~Midiprepped~~	+	\| K143066 \|\| YtvA - Terminator \|\| Sent for Sequencing
	\|}		\|}

	{{Imperial/EndPage\|Major_Results\|Dry_Lab}}		{{Imperial/EndPage\|Major_Results\|Dry_Lab}}

Chris D Hirst at 14:26, 20 October 2008

2008-10-20T14:26:21Z

←Older revision		Revision as of 14:26, 20 October 2008
Line 44:		Line 44:
	\|- style=background:silver		\|- style=background:silver
	\| K143002 \|\| 3' AmyE Integration Sequence \|\| Sequenced		\| K143002 \|\| 3' AmyE Integration Sequence \|\| Sequenced
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:#cc4400
-	\| K143005 \|\| 5' EpsE Integration Sequence \|\| ~~PCR Verified~~	+	\| K143005 \|\| 5' EpsE Integration Sequence \|\| Midiprepped
	\|- style=background:silver		\|- style=background:silver
	\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced		\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced
Line 64:		Line 64:
	\|- style=background:royalblue		\|- style=background:royalblue
	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed		\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed
-	\|- style=background:~~yellow~~	+	\|- style=background:#cc4400
-	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| ~~Pending~~	+	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Midiprepped
	\|- style=background:silver		\|- style=background:silver
	\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced		\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced
Line 84:		Line 84:
	\|- style=background:yellow		\|- style=background:yellow
	\| K143056 \|\| Pxyl-gsiB \|\| Pending		\| K143056 \|\| Pxyl-gsiB \|\| Pending
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:lawngreen
-	\| K143057 \|\| Pxyl-spoVG \|\| ~~PCR Verified~~	+	\| K143057 \|\| Pxyl-spoVG \|\| Sent for Partial Sequencing
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143058 \|\| Pctc-gsiB \|\| ~~Sent for Sequencing~~	+	\| K143058 \|\| Pctc-gsiB \|\| Sequenced
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143059 \|\| Pctc-spoVG \|\| ~~Sent for Sequencing~~	+	\| K143059 \|\| Pctc-spoVG \|\| Sequenced
	\|- style=background:mediumslateblue		\|- style=background:mediumslateblue
	\| K143060 \|\| PgsiB-gsiB \|\| PCR Verified		\| K143060 \|\| PgsiB-gsiB \|\| PCR Verified
Line 96:		Line 96:
	\|- style=background:royalblue		\|- style=background:royalblue
	\| K143062 \|\| LacI - Terminator \|\| Transformed		\| K143062 \|\| LacI - Terminator \|\| Transformed
-	\|- style=background:~~royalblue~~	+	\|- style=background:yellow
-	\| K143063 \|\| XylR - Terminator \|\| ~~Transformed~~	+	\| K143063 \|\| XylR - Terminator \|\| Pending
	\|- style=background:silver		\|- style=background:silver
	\| K143064 \|\| CAT - Terminator \|\| Sequenced		\| K143064 \|\| CAT - Terminator \|\| Sequenced
-	\|- style=background:~~royalblue~~	+	\|- style=background:#cc4400
-	\| K143065 \|\| Aad9 - Terminator \|\| ~~Transformed?~~	+	\| K143065 \|\| Aad9 - Terminator \|\| Midiprepped
-	\|- style=background:~~royalblue~~	+	\|- style=background:#cc4400
-	\| K143066 \|\| YtvA - Terminator \|\| ~~Transformed?~~	+	\| K143066 \|\| YtvA - Terminator \|\| Midiprepped
	\|}		\|}

	{{Imperial/EndPage\|Major_Results\|Dry_Lab}}		{{Imperial/EndPage\|Major_Results\|Dry_Lab}}

Chris D Hirst at 19:57, 16 October 2008

2008-10-16T19:57:16Z

←Older revision		Revision as of 19:57, 16 October 2008
Line 50:		Line 50:
	\|- style=background:yellow		\|- style=background:yellow
	\| K143008 \|\| 5' PyrD Integration Sequence \|\| Pending		\| K143008 \|\| 5' PyrD Integration Sequence \|\| Pending
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143009 \|\| 3' PyrD Integration Sequence \|\| ~~Sent for Sequencing~~	+	\| K143009 \|\| 3' PyrD Integration Sequence \|\| Sequenced
	\|- style=background:silver		\|- style=background:silver
	\| K143031 \|\| Aad9 Spectinomycin Resistance Gene \|\| Sequenced		\| K143031 \|\| Aad9 Spectinomycin Resistance Gene \|\| Sequenced
Line 60:		Line 60:
	\|- style=background:#cc4400		\|- style=background:#cc4400
	\| K143034 \|\| LipA-EAK16 Fusion \|\| Partially Sequenced		\| K143034 \|\| LipA-EAK16 Fusion \|\| Partially Sequenced
-	~~\|- style=background:#cc4400~~
-	~~\| K143035 \|\| LipA-Human Elastin Fusion \|\| Sent for Sequencing~~
-	~~\|- style=background:#cc4400~~
-	~~\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Sent for Sequencing~~
-	~~\|- style=background:#cc4400~~
-	~~\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Sent for Sequencing~~
	\|- style=background:silver		\|- style=background:silver
-	\| ~~K143038~~ \|\| ~~SacB~~-~~EAK16~~ Fusion \|\| Sequenced	+	\| K143035 \|\| LipA-Human Elastin Fusion \|\| Sequenced
		+	\|- style=background:royalblue
		+	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Transformed
	\|- style=background:yellow		\|- style=background:yellow
-	\| K143039 \|\| SacB-Human Elastin Fusion \|\| ~~Pending~~	+	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Pending
		+	\|- style=background:silver
		+	\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced
		+	\|- style=background:#cc4400
		+	\| K143039 \|\| SacB-Human Elastin Fusion \|\| Sent for Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143050 \|\| P43-gsiB \|\| Sequenced		\| K143050 \|\| P43-gsiB \|\| Sequenced
Line 81:		Line 81:
	\| K143054 \|\| Phyperspank-gsiB \|\| Pending		\| K143054 \|\| Phyperspank-gsiB \|\| Pending
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143055 \|\| Phyperspank-spoVG \|\| ~~Sent for Sequencing~~	+	\| K143055 \|\| Phyperspank-spoVG \|\| Sequenced
	\|- style=background:yellow		\|- style=background:yellow
	\| K143056 \|\| Pxyl-gsiB \|\| Pending		\| K143056 \|\| Pxyl-gsiB \|\| Pending
-	\|- style=background:~~yellow~~	+	\|- style=background:mediumslateblue
-	\| K143057 \|\| Pxyl-spoVG \|\| ~~Pending~~	+	\| K143057 \|\| Pxyl-spoVG \|\| PCR Verified
	\|- style=background:#cc4400		\|- style=background:#cc4400
	\| K143058 \|\| Pctc-gsiB \|\| Sent for Sequencing		\| K143058 \|\| Pctc-gsiB \|\| Sent for Sequencing
	\|- style=background:#cc4400		\|- style=background:#cc4400
	\| K143059 \|\| Pctc-spoVG \|\| Sent for Sequencing		\| K143059 \|\| Pctc-spoVG \|\| Sent for Sequencing
-	\|- style=background:~~lawngreen~~	+	\|- style=background:mediumslateblue
-	\| K143060 \|\| PgsiB-gsiB \|\| ~~Sent for Sequencing~~	+	\| K143060 \|\| PgsiB-gsiB \|\| PCR Verified
	\|- style=background:silver		\|- style=background:silver
	\| K143061 \|\| PgsiB-spoVG \|\| Sequenced		\| K143061 \|\| PgsiB-spoVG \|\| Sequenced
-	\|- style=background:~~yellow~~	+	\|- style=background:royalblue
-	\| K143062 \|\| LacI - Terminator \|\| ~~Pending~~	+	\| K143062 \|\| LacI - Terminator \|\| Transformed
-	\|- style=background:~~yellow~~	+	\|- style=background:royalblue
-	\| K143063 \|\| XylR - Terminator \|\| ~~Pending~~	+	\| K143063 \|\| XylR - Terminator \|\| Transformed
	\|- style=background:silver		\|- style=background:silver
	\| K143064 \|\| CAT - Terminator \|\| Sequenced		\| K143064 \|\| CAT - Terminator \|\| Sequenced
-	\|- style=background:~~yellow~~	+	\|- style=background:royalblue
-	\| K143065 \|\| Aad9 - Terminator \|\| ~~Pending~~	+	\| K143065 \|\| Aad9 - Terminator \|\| Transformed?
		+	\|- style=background:royalblue
		+	\| K143066 \|\| YtvA - Terminator \|\| Transformed?
	\|}		\|}

	{{Imperial/EndPage\|Major_Results\|Dry_Lab}}		{{Imperial/EndPage\|Major_Results\|Dry_Lab}}

Chris D Hirst at 09:47, 15 October 2008

2008-10-15T09:47:08Z

←Older revision		Revision as of 09:47, 15 October 2008
Line 51:		Line 51:
	\| K143008 \|\| 5' PyrD Integration Sequence \|\| Pending		\| K143008 \|\| 5' PyrD Integration Sequence \|\| Pending
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143009 \|\| 3' PyrD Integration Sequence \|\| ~~Midiprepped~~	+	\| K143009 \|\| 3' PyrD Integration Sequence \|\| Sent for Sequencing
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143031 \|\| Aad9 Spectinomycin Resistance Gene \|\| ~~Sent for Sequencing~~	+	\| K143031 \|\| Aad9 Spectinomycin Resistance Gene \|\| Sequenced
	\|- style=background:silver		\|- style=background:silver
	\| K143032 \|\| EpsE Molecular Clutch Gene of ''B. subtilis'' \|\| Sequenced		\| K143032 \|\| EpsE Molecular Clutch Gene of ''B. subtilis'' \|\| Sequenced
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	\|- style=background:#cc4400		\|- style=background:#cc4400
	\| K143034 \|\| LipA-EAK16 Fusion \|\| Partially Sequenced		\| K143034 \|\| LipA-EAK16 Fusion \|\| Partially Sequenced
-	\|- style=background:~~royalblue~~	+	\|- style=background:#cc4400
-	\| K143035 \|\| LipA-Human Elastin Fusion \|\| ~~Transformed~~	+	\| K143035 \|\| LipA-Human Elastin Fusion \|\| Sent for Sequencing
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:#cc4400
-	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| ~~PCR verified~~	+	\| K143036 \|\| Xylose Operon Regulatory Protein \|\| Sent for Sequencing
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:#cc4400
-	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| ~~PCR verified~~	+	\| K143037 \|\| Ytva Blue Light Receptor of ''B. subtilis'' \|\| Sent for Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced		\| K143038 \|\| SacB-EAK16 Fusion \|\| Sequenced
-	\|- style=background:~~maroon~~	+	\|- style=background:yellow
-	\| K143039 \|\| SacB-Human Elastin Fusion \|\| ~~Fails to clone~~	+	\| K143039 \|\| SacB-Human Elastin Fusion \|\| Pending
	\|- style=background:silver		\|- style=background:silver
	\| K143050 \|\| P43-gsiB \|\| Sequenced		\| K143050 \|\| P43-gsiB \|\| Sequenced
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	\|- style=background:yellow		\|- style=background:yellow
	\| K143054 \|\| Phyperspank-gsiB \|\| Pending		\| K143054 \|\| Phyperspank-gsiB \|\| Pending
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:#cc4400
-	\| K143055 \|\| Phyperspank-spoVG \|\| ~~PCR verified~~	+	\| K143055 \|\| Phyperspank-spoVG \|\| Sent for Sequencing
	\|- style=background:yellow		\|- style=background:yellow
	\| K143056 \|\| Pxyl-gsiB \|\| Pending		\| K143056 \|\| Pxyl-gsiB \|\| Pending
Line 87:		Line 87:
	\| K143057 \|\| Pxyl-spoVG \|\| Pending		\| K143057 \|\| Pxyl-spoVG \|\| Pending
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143058 \|\| Pctc-gsiB \|\| ~~Midiprepped~~	+	\| K143058 \|\| Pctc-gsiB \|\| Sent for Sequencing
	\|- style=background:#cc4400		\|- style=background:#cc4400
-	\| K143059 \|\| Pctc-spoVG \|\| ~~Midiprepped~~	+	\| K143059 \|\| Pctc-spoVG \|\| Sent for Sequencing
	\|- style=background:lawngreen		\|- style=background:lawngreen
-	\| K143060 \|\| PgsiB-gsiB \|\| ~~Digest Verified~~	+	\| K143060 \|\| PgsiB-gsiB \|\| Sent for Sequencing
	\|- style=background:silver		\|- style=background:silver
	\| K143061 \|\| PgsiB-spoVG \|\| Sequenced		\| K143061 \|\| PgsiB-spoVG \|\| Sequenced

Chris D Hirst at 11:11, 11 October 2008

2008-10-11T11:11:10Z

←Older revision		Revision as of 11:11, 11 October 2008
Line 46:		Line 46:
	\|- style=background:mediumslateblue		\|- style=background:mediumslateblue
	\| K143005 \|\| 5' EpsE Integration Sequence \|\| PCR Verified		\| K143005 \|\| 5' EpsE Integration Sequence \|\| PCR Verified
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143006 \|\| 3' EpsE Integration Sequence \|\| ~~Sent for Sequencing~~	+	\| K143006 \|\| 3' EpsE Integration Sequence \|\| Sequenced
	\|- style=background:yellow		\|- style=background:yellow
	\| K143008 \|\| 5' PyrD Integration Sequence \|\| Pending		\| K143008 \|\| 5' PyrD Integration Sequence \|\| Pending
-	\|- style=background:~~mediumslateblue~~	+	\|- style=background:#cc4400
-	\| K143009 \|\| 3' PyrD Integration Sequence \|\| ~~PCR verified~~	+	\| K143009 \|\| 3' PyrD Integration Sequence \|\| Midiprepped
	\|- style=background:#cc4400		\|- style=background:#cc4400
	\| K143031 \|\| Aad9 Spectinomycin Resistance Gene \|\| Sent for Sequencing		\| K143031 \|\| Aad9 Spectinomycin Resistance Gene \|\| Sent for Sequencing
Line 70:		Line 70:
	\|- style=background:maroon		\|- style=background:maroon
	\| K143039 \|\| SacB-Human Elastin Fusion \|\| Fails to clone		\| K143039 \|\| SacB-Human Elastin Fusion \|\| Fails to clone
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143050 \|\| P43-gsiB \|\| ~~Sent for Sequencing~~	+	\| K143050 \|\| P43-gsiB \|\| Sequenced
	\|- style=background:silver		\|- style=background:silver
	\| K143051 \|\| P43-spoVG \|\| Sequenced		\| K143051 \|\| P43-spoVG \|\| Sequenced
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	\|- style=background:#cc4400		\|- style=background:#cc4400
	\| K143059 \|\| Pctc-spoVG \|\| Midiprepped		\| K143059 \|\| Pctc-spoVG \|\| Midiprepped
-	\|- style=background:~~red~~	+	\|- style=background:lawngreen
-	\| K143060 \|\| PgsiB-gsiB \|\| ~~Wrong insert cloned~~	+	\| K143060 \|\| PgsiB-gsiB \|\| Digest Verified
	\|- style=background:silver		\|- style=background:silver
	\| K143061 \|\| PgsiB-spoVG \|\| Sequenced		\| K143061 \|\| PgsiB-spoVG \|\| Sequenced
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	\|- style=background:yellow		\|- style=background:yellow
	\| K143063 \|\| XylR - Terminator \|\| Pending		\| K143063 \|\| XylR - Terminator \|\| Pending
-	\|- style=background:~~#cc4400~~	+	\|- style=background:silver
-	\| K143064 \|\| CAT - Terminator \|\| ~~Sent for Sequencing~~	+	\| K143064 \|\| CAT - Terminator \|\| Sequenced
	\|- style=background:yellow		\|- style=background:yellow
	\| K143065 \|\| Aad9 - Terminator \|\| Pending		\| K143065 \|\| Aad9 - Terminator \|\| Pending