IGEM:IMPERIAL/2008/New/BioBricks: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
mNo edit summary
Line 1: Line 1:
{{Imperial/StartPage2}}__NOTOC__
{{Imperial/StartPage2}}__NOTOC__
<br><br>
&nbsp; <font size=6px color=#E5EBFF><b>BioBricks & Characterisation</b></font>
<br><br>




{{Imperial/Box1||Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.
{{Imperial/Box2|BioBricks & Characterisation|Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.


Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.
Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.
Line 13: Line 9:
}}
}}


{{Imperial/Box1||For now, here are the parts we hope to submit to the Registry, they can also be accessed [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup{{equals}}iGEM2008&group{{equals}}Imperial_College  here] and at the bottom of the page is a table showing the current progress on production of each part:
{{Imperial/Box1|Parts Submitted to the Registry|For now, here are the parts we will submit to the Registry. They can also be accessed [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup{{equals}}iGEM2008&group{{equals}}Imperial_College  here].
<br><br>
<br><br>
<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>
<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>
}}
}}


{{Imperial/Box1||Here is a table of the parts currently in production:}}
{{Imperial/Box1|Production Status|
This table shows our progress with each part:}}


{| border="2" cellpadding="2" style="text-align:left" align="center"
{| border="2" cellpadding="2" style="text-align:left" align="center"

Revision as of 05:25, 30 September 2008

<html> <style type="text/css"> .firstHeading {display: none;} </style> </html> <html> <style type="text/css"> 

   table.calendar          { margin:0; padding:2px; }

table.calendar td { margin:0; padding:1px; vertical-align:top; } table.month .heading td { padding:1px; background-color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td { text-align:center; font-size:110%; } table.month td.today { background-color:#3366FF } table.month td { 

   border:2px;
   margin:0;
   padding:0pt 1.5pt;
   font-size:8pt;
   text-align:right;
   background-color:#FFFFFF;
   }
  1. bodyContent table.month a { background:none; padding:0 }

.day-active { font-weight:bold; } .day-empty { color:black; } </style> </html>

<html><script language="JavaScript">

var timeout = 250; var closetimer = 0; var ddmenuitem = 0;

// open hidden layer function mopen(id) { // cancel close timer mcancelclosetime(); // close old layer if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; // get new layer and show it ddmenuitem = document.getElementById(id); ddmenuitem.style.visibility = 'visible'; } // close showed layer function mclose() { if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; } // go close timer function mclosetime() { closetimer = window.setTimeout(mclose, timeout); } // cancel close timer function mcancelclosetime() { if(closetimer) { window.clearTimeout(closetimer); closetimer = null; } } // close layer when click-out //document.onclick = mclose; </script> <table background="http://i59.photobucket.com/albums/g305/Timpski/ToolbarBackground.png" style="color:#ffffff;" link="#ffffff" cellpadding="0" cellspacing="1" border="0" bordercolor="#ffffff" align="center" width="100%"><tr><td colspan="6" class="wetlab"><br><br><br></td></tr> <tr><td align="center" width="10%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Home"> Home </a></ul> </td><td align="center" width="20%" valign="bottom"><ul id="sddm"><a href="#" 

       onclick="mopen('m1')" 
       onmouseover="mopen('m1')" 
       onmouseout="mclosetime()">Biofabricator Subtilis</a>
       <div id="m1" 
           onmouseover="mcancelclosetime()" 
           onmouseout="mclosetime()">
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
       </div></ul>

</td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#" 

       onclick="mopen('m2')" 
       onmouseover="mopen('m2')" 
       onmouseout="mclosetime()">Wet Lab</a>
       <div id="m2" 
           onmouseover="mcancelclosetime()" 
           onmouseout="mclosetime()">
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Wet_Lab">Wet Lab Hub</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Cloning_Strategy">Cloning Strategy</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
       </div></ul>

</td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#" 

       onclick="mopen('m3')" 
       onmouseover="mopen('m3')" 
       onmouseout="mclosetime()">Dry Lab</a>
       <div id="m3" 
           onmouseover="mcancelclosetime()" 
           onmouseout="mclosetime()">
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Dry_Lab">Dry Lab Hub</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Growth_Curve">Growth Curves</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Genetic_Circuit">Genetic Circuits</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
       </div></ul>

</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

<html><style type="text/css">

div.Section { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; background-image: url(http://openwetware.org/images/a/a0/Background.PNG); background-size: 100%; background-origin: content; }

/* Text (paragraphs) */ div.Section p { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; margin-top:0px; margin-left:30px; margin-right:30px; }

/* Headings */ div.Section h1 { font:22pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; color:#3366FF; font-weight:bold; }

/* Subheadings */ div.Section h2 { font:18pt Calibri, Verdana, Arial, Geneva, sans-serif; color:#3366FF; margin-left:5px; font-weight:bold; }

/* Subsubheadings */ div.Section h3 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#E5EBFF; margin-left:10px; font-weight:bold; }

/* Subsubsubheadings */ div.Section h4 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#2B48B3; margin-left:10px; font-weight:bold; }

/* Subsubsubsubheadings */ div.Section h5 { font:12pt Calibri, Verdana, Arial, sans-serif; color:#3366FF; margin-left:20px; }

/* References */ div.Section h6 { font:12pt Calibri, Verdana, Arial, sans-serif; font-weight:bold; font-style:italic; color:#3366FF; margin-left:25px; }

/* Hyperlinks */ div.Section a { }

div.Section a:hover { }

/* Tables */ div.Section td { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; vertical-align:top; padding:2px 4px 2px 4px; }

/* Lists */ div.Section li { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; margin-top:0px; margin-left:30px; margin-right:0px; }

/* TOC stuff */ table.toc { margin-left:10px; }

table.toc li { font: 11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align: justify; margin-top: 0px; margin-left:2px; margin-right:2px; }

/* [edit] links */ span.editsection { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

span.editsection a { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

span.editsection a:hover { color:#3366FF; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

/* Drop-down Menu */

  1. sddm {

margin: 0; padding: 0; z-index: 30 margin: 0; padding: 0; float: center; font: bold 12pt Calibri, Verdana, Arial, Geneva, sans-serif; border: 0px; list-style: none; }

  1. sddm a {

display: block; margin: 0px 0px 0px 0px; padding: 0 0 12px 0; color: #FFFFFF; text-align: center; text-decoration: none; }

  1. sddm a:hover {

border: 0px }

  1. sddm div {

position: absolute; visibility: hidden; margin: 0; padding: 0; background: #66aadd; border: 1px solid #66aadd } #sddm div a { position: relative; left: 0; display: block; margin: 0; padding: 5px 10px; width: auto; white-space: nowrap; text-align: left; text-decoration: none; background: #FFFFFF; color: #2875DE; font: 11pt Calibri, Verdana, Arial, Geneva, sans-serif } #sddm div a:hover { background: #66aadd; color: #FFFFFF } </style></html>


BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

<html><center><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png">| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>


Parts Submitted to the Registry

For now, here are the parts we will submit to the Registry. They can also be accessed here.

<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>


Production Status

This table shows our progress with each part:


Part Number Part Name Status
K143001 5' AmyE Integration Sequence Midiprepped
K143002 3' AmyE Integration Sequence Midiprepped
K143005 5' EpsE Integration Sequence Awaiting Ligation
K143006 3' EpsE Integration Sequence Awaiting Ligation
K143008 5' PyrD Integration Sequence Awaiting GeneArt delivery
K143009 3' PyrD Integration Sequence Awaiting Ligation
K143010 Promoter ctc for B. subtilis
K143011 Promoter gsiB for B. subtilis
K143012 Promoter veg for B. subtilis
K143013 Promoter 43 for B. subtilis
K143014 Promoter Xyl for B. subtilis
K143015 Promoter hyper-spank for B. subtilis
K143020 GsiB Ribosome Binding Site (RBS) for B. subtilis
K143021 SpoVG Ribosome Binding Site (RBS) for B. subtilis
K143031 Aad9 Spectinomycin Resistance Gene Ligation Failed
K143032 EpsE Molecular Clutch Gene of B. subtilis Midiprepped
K143033 LacI (Lva-, N terminal deletion) PCR verified
K143034 LipA-EAK16 Fusion Midiprepped
K143035 LipA-Human Elastin Fusion Transformed 25/9
K143036 Xylose Operon Regulatory Protein Transformed 29/9
K143037 Ytva Blue Light Receptor of B. subtilis Awaiting Ligation
K143038 SacB-EAK16 Fusion Midiprepped
K143050 P43-gsiB Midiprepped
K143051 P43-spoVG Midiprepped
K143052 Pveg-gsiB Midiprepped
K143053 Pveg-spoVG Midiprepped
K143054 Phyperspank-gsiB Awaiting GeneArt Delivery
K143055 Phyperspank-spoVG Awaiting Ligation
K143056 Pxyl-gsiB Awaiting GeneArt Delivery
K143057 Pxyl-spoVG Awaiting GeneArt Delivery
K143058 Pctc-gsiB Awaiting Ligation
K143059 Pctc-spoVG Awaiting Ligation
K143060 PgsiB-gsiB Midiprepped
K143061 PgsiB-spoVG Midiprepped
K143062 LacI - Terminator Awaiting Components (LacI)
K143063 XylR - Terminator Awaiting Components (XylR)
K143064 CAT - Terminator Awaiting Ligation
K143065 Aad9 - Terminator Awaiting Components (Aad9)


<html><center><table style="color:#ffffff;background-color:#66aadd;" cellpadding="3" cellspacing="1" border="0" bordercolor="#ffffff" align="center"> <tr><td><ul id="sddm"></html>< Previous<html></ul> </td><td><ul id="sddm"><a href="#">Back to top</a></ul> </td><td><ul id="sddm"></html>Next ><html></ul> </td></tr></table> </center></html>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:IMPERIAL/2008/New/BioBricks&oldid=247580"