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{| border="2" cellpadding="2" style="text-align:left" align="left" width="75%"
{| border="2" cellpadding="2" style="text-align:left" align="left" width="75%"
!Part Number !! Part Name !! Status  
!Part Number !! Part Name !! Status  
|- style=background:silver
|- style=background:gold
| K143001 || 5' AmyE Integration Sequence || Sequenced
| K143001 || 5' AmyE Integration Sequence || Submitted
|- style=background:silver
|- style=background:gold
| K143002 || 3' AmyE Integration Sequence || Sequenced
| K143002 || 3' AmyE Integration Sequence || Submitted
|- style=background:mediumslateblue
|- style=background:gold
| K143005 || 5' EpsE Integration Sequence || PCR Verified
| K143005 || 5' EpsE Integration Sequence || Submitted
|- style=background:#cc4400
|- style=background:gold
| K143006 || 3' EpsE Integration Sequence || Sent for Sequencing
| K143006 || 3' EpsE Integration Sequence || Submitted
|- style=background:yellow
| K143008 || 5' PyrD Integration Sequence || Pending
|- style=background:mediumslateblue
| K143009 || 3' PyrD Integration Sequence || PCR verified
|- style=background:#cc4400
| K143031 || Aad9 Spectinomycin Resistance Gene || Sent for Sequencing
|- style=background:silver
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
|- style=background:silver
| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || Sequenced
|- style=background:#cc4400
| K143034 || LipA-EAK16 Fusion || Partially Sequenced
|- style=background:royalblue
|- style=background:royalblue
| K143035 || LipA-Human Elastin Fusion || Transformed
| K143008 || 5' PyrD Integration Sequence || Delayed
|- style=background:mediumslateblue
|- style=background:gold
| K143036 || Xylose Operon Regulatory Protein || PCR verified
| K143009 || 3' PyrD Integration Sequence || Submitted
|- style=background:mediumslateblue
|- style=background:gold
| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || PCR verified
| K143031 || Aad9 Spectinomycin Resistance Gene || Submitted
|- style=background:silver
|- style=background:gold
| K143038 || SacB-EAK16 Fusion || Sequenced
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Submitted
|- style=background:maroon
|- style=background:gold
| K143039 || SacB-Human Elastin Fusion || Fails to clone
| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || Submitted
|- style=background:#cc4400
|- style=background:gold
| K143050 || P43-gsiB || Sent for Sequencing
| K143034 || LipA-EAK16 Fusion || Submitted
|- style=background:silver
|- style=background:gold
| K143051 || P43-spoVG || Sequenced
| K143035 || LipA-Human Elastin Fusion || Submitted
|- style=background:silver
|- style=background:royalblue
| K143052 || Pveg-gsiB || Sequenced
| K143036 || Xylose Operon Regulatory Protein || Delayed
|- style=background:silver
|- style=background:gold
| K143053 || Pveg-spoVG || Sequenced
| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || Submitted
|- style=background:yellow
|- style=background:gold
| K143054 || Phyperspank-gsiB || Pending
| K143038 || SacB-EAK16 Fusion || Submitted
|- style=background:mediumslateblue
|- style=background:gold
| K143055 || Phyperspank-spoVG || PCR verified
| K143039 || SacB-Human Elastin Fusion || Submitted
|- style=background:yellow
|- style=background:gold
| K143056 || Pxyl-gsiB || Pending
| K143050 || P43-gsiB || Submitted
|- style=background:yellow
|- style=background:gold
| K143057 || Pxyl-spoVG || Pending
| K143051 || P43-spoVG || Submitted
|- style=background:#cc4400
|- style=background:gold
| K143058 || Pctc-gsiB || Midiprepped
| K143052 || Pveg-gsiB || Submitted
|- style=background:#cc4400
|- style=background:gold
| K143059 || Pctc-spoVG || Midiprepped
| K143053 || Pveg-spoVG || Submitted
|- style=background:red
|- style=background:gold
| K143060 || PgsiB-gsiB || Wrong insert cloned
| K143054 || Phyperspank-gsiB || Submitted
|- style=background:silver
|- style=background:gold
| K143061 || PgsiB-spoVG || Sequenced
| K143055 || Phyperspank-spoVG || Submitted
|- style=background:yellow
|- style=background:gold
| K143062 || LacI - Terminator || Pending
| K143056 || Pxyl-gsiB || Submitted
|- style=background:yellow
|- style=background:gold
| K143063 || XylR - Terminator || Pending
| K143057 || Pxyl-spoVG || Submitted
|- style=background:#cc4400
|- style=background:gold
| K143064 || CAT - Terminator || Sent for Sequencing
| K143058 || Pctc-gsiB || Submitted
|- style=background:yellow
|- style=background:gold
| K143065 || Aad9 - Terminator || Pending
| K143059 || Pctc-spoVG || Submitted
|- style=background:gold
| K143060 || PgsiB-gsiB || Submitted
|- style=background:gold
| K143061 || PgsiB-spoVG || Submitted
|- style=background:gold
| K143062 || LacI - Terminator || Submitted
|- style=background:royalblue
| K143063 || XylR - Terminator || Delayed
|- style=background:gold
| K143064 || CAT - Terminator || Submitted
|- style=background:gold
| K143065 || Aad9 - Terminator || Submitted
|- style=background:gold
| K143066 || YtvA - Terminator || Submitted
|- style=background:gold
| K143067 || AmyE integratable PoPS generator (P43-gsiB) || Submitted
|- style=background:gold
| K143068 || AmyE integratable PoPS generator (P43-spoVG) || Submitted
|- style=background:gold
| K143069 || AmyE integratable PoPS generator (Pveg-gsiB) || Submitted
|- style=background:gold
| K143070 || AmyE integratable PoPS generator (Pveg-spoVG) || Submitted
|- style=background:gold
| K143071 || AmyE integratable PoPS generator (P43-gsiB) (with CmR) || Submitted
|- style=background:gold
| K143072 || AmyE integratable PoPS generator (P43-spoVG) (with CmR) || Submitted
|- style=background:royalblue
| K143073 || AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) || Delayed
|- style=background:gold
| K143074 || AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) || Submitted
|- style=background:gold
| K143075 || B. subtilis AmyE locus GFP output promoter and RBS tester || Submitted
|- style=background:gold
| K143076 || B. subtilis AmyE locus RFP output promoter and RBS tester || Submitted
|- style=background:gold
| K143077 || P43-gsiB GFP expression construct  || Submitted
|- style=background:gold
| K143078 || P43-spoVG GFP expression construct  || Submitted
|- style=background:gold
| K143079 || Pveg-spoVG GFP expression construct  || Submitted
|- style=background:gold
| K143080 || P43-gsiB RFP expression construct  || Submitted
|- style=background:gold
| K143081 || P43-spoVG RFP expression construct  || Submitted
|- style=background:gold
| K143082 || Pveg-spoVG RFP expression construct  || Submitted
|}
|}


{{Imperial/EndPage|Major_Results|Dry_Lab}}
{{Imperial/EndPage|Major_Results|Dry_Lab}}

Latest revision as of 18:56, 28 October 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

<html><center><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png">| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>


Parts Submitted to the Registry

For now, here are the parts we will submit to the Registry. They can also be accessed here.

<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>


Production Status

This table shows our progress with each part:


Key
Submitted
Sequenced
Midiprepped
Fully verified
Partially Verified
Transformed
Ligation pending/Failed
Awaiting GeneArt
Cloning difficulties
Part Number Part Name Status
K143001 5' AmyE Integration Sequence Submitted
K143002 3' AmyE Integration Sequence Submitted
K143005 5' EpsE Integration Sequence Submitted
K143006 3' EpsE Integration Sequence Submitted
K143008 5' PyrD Integration Sequence Delayed
K143009 3' PyrD Integration Sequence Submitted
K143031 Aad9 Spectinomycin Resistance Gene Submitted
K143032 EpsE Molecular Clutch Gene of B. subtilis Submitted
K143033 LacI (Lva-, N terminal deletion) Submitted
K143034 LipA-EAK16 Fusion Submitted
K143035 LipA-Human Elastin Fusion Submitted
K143036 Xylose Operon Regulatory Protein Delayed
K143037 Ytva Blue Light Receptor of B. subtilis Submitted
K143038 SacB-EAK16 Fusion Submitted
K143039 SacB-Human Elastin Fusion Submitted
K143050 P43-gsiB Submitted
K143051 P43-spoVG Submitted
K143052 Pveg-gsiB Submitted
K143053 Pveg-spoVG Submitted
K143054 Phyperspank-gsiB Submitted
K143055 Phyperspank-spoVG Submitted
K143056 Pxyl-gsiB Submitted
K143057 Pxyl-spoVG Submitted
K143058 Pctc-gsiB Submitted
K143059 Pctc-spoVG Submitted
K143060 PgsiB-gsiB Submitted
K143061 PgsiB-spoVG Submitted
K143062 LacI - Terminator Submitted
K143063 XylR - Terminator Delayed
K143064 CAT - Terminator Submitted
K143065 Aad9 - Terminator Submitted
K143066 YtvA - Terminator Submitted
K143067 AmyE integratable PoPS generator (P43-gsiB) Submitted
K143068 AmyE integratable PoPS generator (P43-spoVG) Submitted
K143069 AmyE integratable PoPS generator (Pveg-gsiB) Submitted
K143070 AmyE integratable PoPS generator (Pveg-spoVG) Submitted
K143071 AmyE integratable PoPS generator (P43-gsiB) (with CmR) Submitted
K143072 AmyE integratable PoPS generator (P43-spoVG) (with CmR) Submitted
K143073 AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) Delayed
K143074 AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) Submitted
K143075 B. subtilis AmyE locus GFP output promoter and RBS tester Submitted
K143076 B. subtilis AmyE locus RFP output promoter and RBS tester Submitted
K143077 P43-gsiB GFP expression construct Submitted
K143078 P43-spoVG GFP expression construct Submitted
K143079 Pveg-spoVG GFP expression construct Submitted
K143080 P43-gsiB RFP expression construct Submitted
K143081 P43-spoVG RFP expression construct Submitted
K143082 Pveg-spoVG RFP expression construct Submitted


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