IGEM:IMPERIAL/2007/Projects/pH Buffering Machine/Design

pH Buffering Machine: Design

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

List of Parts Required

• From the registry
  • BBa_J23100: Constitutive promoter
  • BBa_C0012: Coding region for LacI
  • BBa_R0010: pLac promoter – activated by IPTG
  • BBa_E0040: Coding region for GFP
  • BBa_J06504: Coding region for RFP
  • BBa_R0080: pAra promoter – activated by AraC
  • BBa_C0040: Coding region for TetR
  • BBa_R0040: pTet promoter
  • BBa_B0034: RBS
  • BBa_B0015: Double terminator
• Not yet in the registry
  • NC_000913: Coding region for CadC
  • M67452: pCad promoter
  • Coding region for HrpR
  • Coding region for HrpS
  • PhrpL promoter
  • NC_002655: Coding region for urease
  • NC_007946: Coding region for lactate dehydrogenase

Chassis Selection

• E.coli K12 (strain K-12; substrain MG1655]