IGEM:IMPERIAL/2006/project/popsblocker - Revision history

Vincent Rouilly: /* Part interface */

Vincent Rouilly — Mon, 30 Oct 2006 16:47:24 GMT

Part interface

←Older revision		Revision as of 16:47, 30 October 2006
Line 36:		Line 36:
	[[Image:IGEM_IMPERIAL_Methodology_Specifications_Cre.PNG\|right\|350px]]		[[Image:IGEM_IMPERIAL_Methodology_Specifications_Cre.PNG\|right\|350px]]
	\|}		\|}
		+
		+	<html>
		+	<!-- Start of StatCounter Code -->
		+	<script type="text/javascript" language="javascript">
		+	var sc_project=1999441;
		+	var sc_invisible=1;
		+	var sc_partition=18;
		+	var sc_security="18996820";
		+	</script>
		+
		+	<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&java=0&security=18996820&invisible=1" alt="website statistics" border="0"></a> </noscript>
		+	<!-- End of StatCounter Code -->
		+	</html>

Jonny at 19:36, 29 October 2006

Jonny — Sun, 29 Oct 2006 19:36:17 GMT

←Older revision		Revision as of 19:36, 29 October 2006
Line 14:		Line 14:
	==Motivations==		==Motivations==

-	*We wanted a way of controlling the activation of the positive-feedback loop in our predator-prey based oscillator.	+	*We wanted a way of controlling the activation of the positive-feedback loop in our predator-prey based oscillator. The idea was to use some kind of switch, and seeing as the available switches in the registry all had certain drawbacks, we went about designing our own and what we came up with was the Cre-LoxP system
-	*The idea was to use some kind of switch, and seeing as the available switches in the registry all had certain drawbacks, we went about designing our own.	+
-	*The idea we came up with was the Cre-LoxP system:	+
-		+
	*The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.		*The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.

Jonny at 19:35, 29 October 2006

Jonny — Sun, 29 Oct 2006 19:35:22 GMT

←Older revision		Revision as of 19:35, 29 October 2006
Line 13:		Line 13:

	==Motivations==		==Motivations==
		+
		+	*We wanted a way of controlling the activation of the positive-feedback loop in our predator-prey based oscillator.
		+	*The idea was to use some kind of switch, and seeing as the available switches in the registry all had certain drawbacks, we went about designing our own.
		+	*The idea we came up with was the Cre-LoxP system:

	*The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.		*The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.

Jonny at 20:39, 26 October 2006

Jonny — Thu, 26 Oct 2006 20:39:55 GMT

←Older revision		Revision as of 20:39, 26 October 2006
Line 9:		Line 9:
	</div>		</div>
	<br style="clear:both">		<br style="clear:both">
-
-

	===Cre-LoxP System===		===Cre-LoxP System===

Jonny at 20:39, 26 October 2006

Jonny — Thu, 26 Oct 2006 20:39:20 GMT

←Older revision		Revision as of 20:39, 26 October 2006
Line 16:		Line 16:
	==Motivations==		==Motivations==

-	:The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.	+	*The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.

-	In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method.	+	*In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method.

	==Part interface==		==Part interface==

Jonny at 20:38, 26 October 2006

Jonny — Thu, 26 Oct 2006 20:38:49 GMT

←Older revision		Revision as of 20:38, 26 October 2006
Line 16:		Line 16:
	==Motivations==		==Motivations==

-	The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.	+	:The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.
		+
	In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method.		In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method.

Jonny at 20:38, 26 October 2006

Jonny — Thu, 26 Oct 2006 20:38:18 GMT

←Older revision		Revision as of 20:38, 26 October 2006
Line 16:		Line 16:
	==Motivations==		==Motivations==

-	The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch controlled by the activity of the promoter in front of the Cre enzyme.	+	The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.
-		+	In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method.
-	*Using Cre recombinase as a switch appears to be~~, in theory,~~ a very efficient and solid method.	+

	==Part interface==		==Part interface==

Jonny at 20:34, 26 October 2006

Jonny — Thu, 26 Oct 2006 20:34:27 GMT

←Older revision		Revision as of 20:34, 26 October 2006
Line 36:		Line 36:
	\|\|		\|\|
	[[Image:IGEM_IMPERIAL_Methodology_Specifications_Cre.PNG\|right\|350px]]		[[Image:IGEM_IMPERIAL_Methodology_Specifications_Cre.PNG\|right\|350px]]
-	\|}
-
-
-	~~==Expected behavior and performances==~~
-
-	~~(try to write your specifications in tables. Here are just 2 examples)~~
-	~~{\| border="1" width="100%"~~
-	~~Interface specifiactions~~
-	\|-
-	~~! style="background:lightgrey"\|Characteristics !! Input 1 !! Input 2 !! Input 3 !! Output 1 !! Output 2~~
-	\|-
-	~~\| Range \|\| [X1,Y1] \|\| [X2,Y2] \|\| [X3,Y3] \|\| [X4,Y4] \|\| [X5,Y5]~~
-	\|-
-	~~\| Tolerance \|\| T1 \|\|T2 \|\| T3 \|\| T4\|\| T5~~
-	\|-
-	~~\| Transfer function \|\| n/a \|\| n/a \|\| n/a \|\| insert image \|\| insert image~~
-	\|-
-	~~\| Latency\|\| \|\| \|\| \|\| \|\|~~
-	\|}
-
-
-	~~{\| border="1" width="100%"~~
-	~~System level specifications~~
-	\|-
-	~~! style="background:lightgrey" width="100pt" \|Properties !! Comments~~
-	\|-
-	~~\| Range\|\|~~
-	\|-
-	~~\| Transfer function \|\|~~
-	\|-
-	~~\| Latency \|\|~~
-	\|-
-	~~\| Robustness \|\|~~
-	\|-
-	~~\| Variability \|\|~~
-	\|-
-	~~\| properties 3 \|\|~~
-	\|-
-	~~\| properties 4 \|\|~~
-
	\|}		\|}

Jonny at 07:23, 23 October 2006

Jonny — Mon, 23 Oct 2006 07:23:28 GMT

←Older revision		Revision as of 07:23, 23 October 2006
Line 31:		Line 31:
	\|-		\|-
	\| Cre \|\|rowspan = "2"\| Reporter		\| Cre \|\|rowspan = "2"\| Reporter
		+	\|-
	\| Pops		\| Pops
	\|}		\|}

Jonny at 07:20, 23 October 2006

Jonny — Mon, 23 Oct 2006 07:20:24 GMT

←Older revision		Revision as of 07:20, 23 October 2006
Line 22:		Line 22:
	==Part interface==		==Part interface==

-	~~(describe your black-box approach, define properties of inputs/outputs)~~	+
	{\| border="0" width="100% align="center"		{\| border="0" width="100% align="center"
	\|-		\|-
Line 30:		Line 30:
	! style="background:#66CC99"\| INPUTS !!style="background:#FF3333"\| OUTPUTS		! style="background:#66CC99"\| INPUTS !!style="background:#FF3333"\| OUTPUTS
	\|-		\|-
-	\| ~~input 1 \|\| output 1~~	+	\| Cre \|\|rowspan = "2"\| Reporter
-	\|-	+	\| Pops
-	~~\| input 2~~ \|\|rowspan = "2" \| ~~output 2~~	+
-	\|-	+
-	\| ~~input 3~~	+
	\|}		\|}
	\|\|		\|\|
	[[Image:IGEM_IMPERIAL_Methodology_Specifications_Cre.PNG\|right\|350px]]		[[Image:IGEM_IMPERIAL_Methodology_Specifications_Cre.PNG\|right\|350px]]
	\|}		\|}
-	~~(don't forget to insert a relevant image of your black box.)~~	+

	==Expected behavior and performances==		==Expected behavior and performances==
Line 79:		Line 76:

	\|}		\|}
-
-	~~==Open issues==~~