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Revision as of 13:53, 22 October 2006
The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part J37015. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch controlled by the activity of the promoter in front of the Cre enzyme.
- Using Cre recombinase as a switch appears to be, in theory, a very efficient and solid method.
(describe your black-box approach, define properties of inputs/outputs)
(don't forget to insert a relevant image of your black box.)
Expected behavior and performances
(try to write your specifications in tables. Here are just 2 examples)
|Characteristics||Input 1||Input 2||Input 3||Output 1||Output 2|
|Transfer function||n/a||n/a||n/a||insert image||insert image|