IGEM:IMPERIAL/2006/project/parts

Sandbox

Test Constructs

• <bbpart>BBa_J37027</bbpart> Cre Lox - Excision characterisation
  • Cre Lox Protocol

• <bbpart>T9002</bbpart> - Prey Cell Transfer Function Characterisation
  • T9002 Protocol
  • T9002 Experimental Data
  • Transfer Function Standard Data Spreadsheet

• AHL assay based on T9002
  • AHL assay Protocol To be finalized

• <bbpart>J37015</bbpart> - Prey Cell Positive Feedback Loop Characterisation
  • J37015 Protocol
  • IGEM:IMPERIAL/2006/Protocols/J37015sevenhour New Re-done to use the chemostat
  • J37015 Experimental Data

• <bbpart>J37016</bbpart> - Predator Cell Transfer Function Characterisation
  • J37016 Experimental Data
  • Transfer Function Standard Data Spreadsheet

• <bbpart>J37022</bbpart> - Predator Cell AiiA Characterisation
  • J37022 Experimental Data

• <bbpart>I13208</bbpart> - AiiA Zinc Hypothesis Test - No longer needed
  • I13208 Experimental Data

• <bbpart>J37020</bbpart> - Monocistronic Predator Cell Test Construct
  • J37020 Experimental Data
  • Transfer Function Standard Data Spreadsheet

• <bbpart>S01656</bbpart> - AiiA test construct (IPTG induced, +LVA, -FLAG)
  • S01656 Experimental Data

Miscellaneous Testing

• Abs490 OD600 Calibration

• Testing OD on diferent growth Media

• Monitoring cell population in mixed culture. More information can be found here