IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct: Difference between revisions
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==Motivations== | ==Motivations== | ||
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==Expected Behavior and Performance== | |||
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|Output Range|| colspan="2" | Output range of the PoPs can be any range, needs to be measurable with Wallac Victor III Fluorimeter. | |Output Range|| colspan="2" | Output range of the PoPs can be any range, needs to be measurable with Wallac Victor III Fluorimeter. | ||
|- | |- | ||
| Transfer function(s) || [[Image: | | Transfer function(s) || [[Image:TransferFunctPrey.png|250px]] | ||
|- | |- | ||
| Latency || colspan="2" | to be characterized | | Latency || colspan="2" | to be characterized | ||
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| Robustness || colspan="2" | ... | | Robustness || colspan="2" | ... | ||
|- | |- | ||
| Variability || colspan="2" | Variability at the population level (day to day | | Variability || colspan="2" | Variability at the population level (day to day) should be as low as possible (10%) | ||
|} | |} | ||
==Open issues== | ==Open issues== | ||
*list known issues | *list known issues |
Latest revision as of 08:42, 28 October 2006
Super Parts | Prey Construct | ||
---|---|---|---|
Actual Part | T9002: Test Sensing Prey Construct | ||
Sub Parts | F2620 | intermediate_part | intermediate_part |
Motivations
- This testing part, the Test Sensing Prey Construct, is representing the sensitivity of the prey cell towards the prey molecule (AHL)
- This part allows us to quantise the activation of gene expression by different concentrations of AHL and is used to assay AHL
- This testing part allows us to investigate three parameters:
- How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
- How much AHL is present, by referring to the transfer function
- How LuxR levels effect gene activation
Part interface
|
Expected Behavior and Performance
System level specificationsOpen issues
- list known issues