IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct: Difference between revisions
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!style="background:#FF9966" height="25pt" width="80pt"|Actual Part | !style="background:#FF9966" height="25pt" width="80pt"|Actual Part | ||
!colspan="3"| [[Image:T9002.JPG|350px]] | !colspan="3"| [[Image:T9002.JPG|350px]]<br><font color=#FF9966> <big>T9002: Test Sensing Prey Construct</big></font color> | ||
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! height="25pt" width="80pt"|Sub Parts | ! height="25pt" width="80pt"|Sub Parts | ||
![[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| | ![[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part/F2620| F2620]] | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]] | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]] | ||
! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]] | ! [[IGEM:IMPERIAL/2006/project/Oscillator/project browser/intermediate_part| intermediate_part]] | ||
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==Motivations== | ==Motivations== | ||
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==Part interface== | ==Part interface== | ||
{| border="0" width="100% align="center" | {| border="0" width="100% align="center" | ||
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | ||
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| | | Prey Molecule || PoPs | ||
|} | |} | ||
|| | || | ||
[[Image: | [[Image:PreySensingTestConstruct.png|right|350px]] | ||
|} | |} | ||
==Expected Behavior and Performance== | |||
{| border="1" width="100%" | {| border="1" width="100%" | ||
System level specifications | System level specifications | ||
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! style="background:lightgrey" width="100pt" |Properties !! | ! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications | ||
|- | |- | ||
| | | Input Range(s)|| colspan="2" | Input range of the prey should cover the total dynamic range of the prey. | ||
|- | |- | ||
| | |Output Range|| colspan="2" | Output range of the PoPs can be any range, needs to be measurable with Wallac Victor III Fluorimeter. | ||
|- | |- | ||
| | | Transfer function(s) || [[Image:TransferFunctPrey.png|250px]] | ||
|- | |- | ||
| | | Latency || colspan="2" | to be characterized | ||
|- | |- | ||
| | | Robustness || colspan="2" | ... | ||
|- | |- | ||
| | | Variability || colspan="2" | Variability at the population level (day to day) should be as low as possible (10%) | ||
|} | |} | ||
==Open issues== | ==Open issues== | ||
*list known issues | *list known issues |
Latest revision as of 08:42, 28 October 2006
Super Parts | Prey Construct | ||
---|---|---|---|
Actual Part | T9002: Test Sensing Prey Construct | ||
Sub Parts | F2620 | intermediate_part | intermediate_part |
Motivations
- This testing part, the Test Sensing Prey Construct, is representing the sensitivity of the prey cell towards the prey molecule (AHL)
- This part allows us to quantise the activation of gene expression by different concentrations of AHL and is used to assay AHL
- This testing part allows us to investigate three parameters:
- How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
- How much AHL is present, by referring to the transfer function
- How LuxR levels effect gene activation
Part interface
|
Expected Behavior and Performance
System level specificationsOpen issues
- list known issues