The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
The Aiia gene has been replaced with GFP to allow direct measurement of protein production
From this data we can create a transfer function where AHL is the input and GFP the output
Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
The part will be used to form the sensing part of the final predator construct
Parts Interface
INPUTS
OUTPUTS
AHL prey molecule
GFP
Predator construct at the system level.
INPUTS
OUTPUTS
AHL prey molecule
GFP
Predator construct at the device level.
Expected behavior and performances
System level specifications
Properties
Specifications
Input Range(s)
This test should determine the input range of the Predator test construct. A greater range of AHL concentrations than we expect the predator cell to respond to have been added.
Output Range(s)
Testing this part will determine the output range
Output range of the predator to be defined.
Transfer function(s)
Robustness
Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
Variability
Variability at the device population level (day to day, device to device) should be as low as possible.
