IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct: Difference between revisions

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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS  
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS  
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| <center>AHL<br>prey molecule</center> || <center>GFP</center>
| AHL || AHL-lactonase & fluctuating [AHL]
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[[Image:IGEM IMPERIAL PreadatorConstructSystemIcon.png|thumbnail|right|350px|Predator construct at the system level.]]  
[[Image:PredatorAtDeviceLevel.png|thumbnail|right|350px|Predator construct at the device level.]]
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{| border="1" cellpadding="15" width="100%"
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS
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| AHL || AHL-lactonase
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[[Image:Predsenspart.png|thumbnail|right|350px|Predator sensing test construct.]]
{| border="0" width="100% align="center"
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! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS  
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS  
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| <center>AHL<br>prey molecule</center> || <center>GFP</center>
| AHL || GFP
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|}
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[[Image:PredatorAtDeviceLevel.png|thumbnail|right|350px|Predator construct at the device level.]]  
[[Image:Predsensingblackbox.png|thumbnail|right|350px|Predator sensing test construct.]]
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==Expected behavior and performances==
==Expected behavior and performances==
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! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications
! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications
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| Input Range(s)|| colspan="2" | This test should determine the input range of the Predator test construct. A greater range of AHL concentrations than we expect the predator cell to respond to have been added.
| Input Range(s)|| colspan="2" | [0.01nm,10000nm]
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| rowspan="2"| Output Range(s)|| colspan="2" | Testing this part will determine the output range
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| colspan="2" | Output range of the predator to be defined.
| Output Range(s)|| colspan="2" | <font color=red>'''?'''</font>
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| Transfer function(s) || [[image:Transfer_Function_Predator_Sensor.JPG|250px]]  
| Transfer function(s) || [[Image:016transfercurve.png|250px]]  
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| Robustness || colspan="2" | Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
| Robustness || colspan="2" | Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.

Latest revision as of 07:21, 29 October 2006

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Motivation

  • The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
  • The Aiia gene has been replaced with GFP to allow direct measurement of protein production
  • From this data we can create a transfer function where AHL is the input and GFP the output
  • Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
  • The part will be used to form the sensing part of the final predator construct

Parts Interface

INPUTS OUTPUTS
AHL AHL-lactonase & fluctuating [AHL]
Predator construct at the device level.
INPUTS OUTPUTS
AHL AHL-lactonase
Predator sensing test construct.
INPUTS OUTPUTS
AHL GFP
Predator sensing test construct.

Expected behavior and performances

System level specifications
Properties Specifications
Input Range(s) [0.01nm,10000nm]
Output Range(s) ?
Transfer function(s)
Robustness Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
Variability Variability at the device population level (day to day, device to device) should be as low as possible.
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