IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct: Difference between revisions
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!height="25pt" width="80pt"|Sub Parts | !height="25pt" width="80pt"|Sub Parts | ||
! | ! <bbpart>R0062</bbpart> | ||
! | ! <bbpart>B0034</bbpart> | ||
! | ! <bbpart>C0062</bbpart> | ||
! | ! <bbpart>I13504</bbpart> | ||
|} | |} | ||
<div class="tabs-blue"> | <div class="tabs-blue"> | ||
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<br style="clear:both"> | <br style="clear:both"> | ||
==Motivation== | |||
*The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase | |||
*The Aiia gene has been replaced with GFP to allow direct measurement of protein production | |||
*From this data we can create a transfer function where AHL is the input and GFP the output | |||
*Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator | |||
*The part will be used to form the sensing part of the final predator construct | |||
==Parts Interface== | |||
{| border="0" width="100% align="center" | |||
|- | |||
| | |||
{| border="1" cellpadding="15" width="100%" | |||
|- | |||
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | |||
|- | |||
| AHL || AHL-lactonase & fluctuating [AHL] | |||
|} | |||
|| | |||
[[Image:PredatorAtDeviceLevel.png|thumbnail|right|350px|Predator construct at the device level.]] | |||
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| | |||
{| border="1" cellpadding="15" width="100%" | |||
|- | |||
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | |||
|- | |||
| AHL || AHL-lactonase | |||
|} | |||
|| | |||
[[Image:Predsenspart.png|thumbnail|right|350px|Predator sensing test construct.]] | |||
|- | |||
| | |||
{| border="1" cellpadding="15" width="100%" | |||
|- | |||
! style="background:#66CC99"| INPUTS !!style="background:#FF3333"| OUTPUTS | |||
|- | |||
| AHL || GFP | |||
|} | |||
|| | |||
[[Image:Predsensingblackbox.png|thumbnail|right|350px|Predator sensing test construct.]] | |||
|} | |||
==Expected behavior and performances== | |||
{| border="1" width="100%" | |||
System level specifications | |||
|- | |||
! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications | |||
|- | |||
| Input Range(s)|| colspan="2" | [0.01nm,10000nm] | |||
|- | |||
| Output Range(s)|| colspan="2" | <font color=red>'''?'''</font> | |||
|- | |||
| Transfer function(s) || [[Image:016transfercurve.png|250px]] | |||
|- | |||
| Robustness || colspan="2" | Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications. | |||
|- | |||
| Variability || colspan="2" | Variability at the device population level (day to day, device to device) should be as low as possible. | |||
|} |
Latest revision as of 07:21, 29 October 2006
Super Parts | Predator Construct | |||
---|---|---|---|---|
Actual Part | ||||
Sub Parts | <bbpart>R0062</bbpart> | <bbpart>B0034</bbpart> | <bbpart>C0062</bbpart> | <bbpart>I13504</bbpart> |
Motivation
- The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
- The Aiia gene has been replaced with GFP to allow direct measurement of protein production
- From this data we can create a transfer function where AHL is the input and GFP the output
- Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
- The part will be used to form the sensing part of the final predator construct
Parts Interface
|
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