IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct

Super Parts	Predator Construct
Actual Part
Sub Parts	<bbpart>R0062</bbpart>	<bbpart>B0034</bbpart>	<bbpart>C0062</bbpart>	<bbpart>I13504</bbpart>

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Predator Test Construct

Motivation

The purpouse for building and testing this part is so we can test the predator cell in isolation to determine the Production rates of the proteins involved in the predator cell. The Aiia gene has been replaced with GFP. This allows us to directly mesure the production rate of a protein which is located in the place of GFP in our final system.

From this data we can create a transfer function where AHL is the input and GFP the output. We then make the assumpion that aiiA is transcribed at the same rate as GFP. This allpws us determine paremeters for our model.

• In this part the AiiA gene has been replaced with the reporter gene GFP to allow the measurement of the production of GFP as a function of AHL.
• The resulting transfer curve will be used to extract parameters Vmax and Kd to characterize the part and for use in our modelling.
• The part will form the receiver of the final predator construct

Parts Interface

This Part is designed to mimic the predator sensing part to get parameters for the model. It will give us an idea of the expression of aiiA.

INPUTS OUTPUTS AHL GFP

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Predator construct at the system level.

Predator construct at the device level.

Expected behavior and performances

System level specifications

Properties	Specifications
Input Range(s)	Input range of the prey should at least match the dynamic range of the prey.
Output Range(s)	Output range of the prey should at least match the dynamic range of the prey.
	Output range of the predator to be defined.
Transfer function(s)	Transfer function inspired by the pure Lotka-Volterra equations, growth predator = C*prey_population_*predator_population	Transfer function inspired by the pure Lotka-Volterra equations, prey killing = B*prey_population_*predator_population.
Robustness	Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
Variability	Variability at the device population level (day to day, device to device) should be as low as possible.