IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Design: Difference between revisions
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==Registry== | ==Registry== | ||
*The prey part: [http://parts.mit.edu/registry/index.php/Part:BBa_J37015 J37015 @ MIT Parts Registry] | |||
[[Image:J37015. | [[Image:J37015 logo.png]] | ||
==Design Choices== | ==Design Choices== | ||
Revision as of 17:34, 29 October 2006
| Super Parts | Molecular Prey-Predator Oscillator | ||
|---|---|---|---|
| Actual Part | 
| ||
| Sub Parts | Test Sensing Prey Construct | <bbpart>C0261</bbpart> | <bbpart>I13401</bbpart> |
Registry
- The prey part: J37015 @ MIT Parts Registry
Design Choices
- Design allows for an exponential increase of AHL via a positive feedback loop
- Interaction between LuxR, AHL and promoter controlling expression of LuxI
- Design allows for assesment of status of positive feedback loop via:
- GFP Production - Linked to LuxI expression
- AHL Production - Can be indirectly assessed via T9002 assay
| INPUTS | biological component | comments |
|---|---|---|
| AHL | LuxR + pLuxR | These components sense input and activate output |
| OUTPUTS | biological component | comments |
| AHL | LuxI | LuxI expression causes enzymatic generation of AHL |
