IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation: Difference between revisions

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| <center><bbpart>J37018</bbpart></center> || [[Image:J37018 logo.png]] || No || N/A || N/A
| <center><bbpart>J37018</bbpart></center> || [[Image:J37018 logo.png]] || No || N/A || N/A
|-
|-
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || Forward pSB1(AK3/A3/A2)
| <center><bbpart>J37019</bbpart></center> || [[Image:J37019 part.png]] || Yes || [http://openwetware.org/images/e/e3/J37019_sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
Sequence: TAT TGT CTC ATG AGC GG<br>
Seconday Structure: None
|-
|-
| <center><bbpart>J37023</bbpart></center> || [[Image:J37023 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37023</bbpart></center> || [[Image:J37023 logo.png]] || Yes || [http://openwetware.org/images/b/b3/J37025sequencing.doc Yes] <br> Note: This part was sequenced as a part of J37025 || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
|-
| <center><bbpart>J37024</bbpart></center> || [[Image:J37024 logo.png]] || No || N/A || N/A
| <center><bbpart>J37024</bbpart></center> || [[Image:J37024 logo.png]] || No || N/A || N/A
|-
|-
| <center><bbpart>J37025</bbpart></center> || [[Image:J37025 logo.png]] || No || N/A || N/A
| <center><bbpart>J37025</bbpart></center> || [[Image:J37025 logo.png]] || Yes || [http://openwetware.org/images/b/b3/J37025sequencing.doc Yes] || [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/Primers Primers used]
|-
|-
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || <font color=red>'''?'''</font> || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37031</bbpart></center> || [[Image:J37031 logo.png]] || Yes || No || N/A
|-
|-
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font>
| <center><bbpart>J37033</bbpart></center> || [[Image:J37033 logo.png]] || Yes || No || N/A
|-
|-
| <center><bbpart>J37035</bbpart></center> || [[Image:J37035 logo.png]] || No || N/A || N/A
| <center><bbpart>J37035</bbpart></center> || [[Image:J37035 logo.png]] || No || N/A || N/A

Revision as of 08:12, 30 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Predator Construct Test Killing Predator Construct


Ligation strategy

Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37018</bbpart>
 No N/A N/A
<bbpart>J37019</bbpart>
 Yes Yes Primers used
<bbpart>J37023</bbpart>
 Yes Yes
Note: This part was sequenced as a part of J37025		 Primers used
<bbpart>J37024</bbpart>
 No N/A N/A
<bbpart>J37025</bbpart>
 Yes Yes Primers used
<bbpart>J37031</bbpart>
 Yes No N/A
<bbpart>J37033</bbpart>
 Yes No N/A
<bbpart>J37035</bbpart>
 No N/A N/A
<bbpart>J37036</bbpart>
 No N/A N/A

WetLab work entries

  • the final assembly was not successful
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