IGEM:IMPERIAL/2006/project/Oscillator/project browser

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===Project browser===
===Project browser===
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{| border="1" cellpadding="5"
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{| border="1" cellpadding="5"  
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!width="50"|Final System
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!width="20%" style="background:#2171B8"|<font color=white>Final System</font>
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! colspan="8" width="80%"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System | Molecular Prey-Predator Oscillator ]]
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! colspan="8" width="80%" style="background:#CCCCFF"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Full_System | Molecular Prey-Predator Oscillator ]] <br>[[Image:Oscillator_Icon.PNG|40px]]
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!width="50"|Final Constructs
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!width="20%" style="background:#2171B8"|<font color=white>Final Constructs</font>
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! colspan="3" width="40%"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct | Prey construct ]]  
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! colspan="3" width="40%" style="background:#CCCCFF"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct | Prey construct ]] <br>[[Image:J37015page.JPG‎|180px]]
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! colspan="5" width="40%"|  
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! colspan="5" width="40%" style="background:#CCCCFF"|  
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[[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct | Predator construct ]]  
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[[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct | Predator construct ]] <br> [[Image:J37036 parts pic.PNG|150px]]
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!width="50"|Test Constructs  
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!width="20%" style="background:#2171B8"|<font color=white>Test Constructs </font>
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! colspan="1" width="40%"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Prey_Construct | Test Sensing Prey ]]  
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! colspan="1" width="20%" style="background:#CCCCFF"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Prey_Construct | Test Sensing Prey ]] [[Image:T9002.JPG|130px]]
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! colspan="2" width="40%"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Prey_Construct | Test Prey production ]]  
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! colspan="2" width="20%" style="background:#CCCCFF"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Prey_Construct | Test Prey production ]] [[Image:J37015page.JPG‎|180px]]
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! colspan="3" width="20%"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct | Test Sensing Predator]]  
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! colspan="3" width="20%" style="background:#CCCCFF"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct | Test Sensing Predator]] [[Image:J37016.PNG|130px]]
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! colspan="2" width="20%"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct | Test Killing Predator]]
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! colspan="2" width="20%" style="background:#CCCCFF"| [[IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Killing_Predator_Construct | Test Killing Predator]] [[Image:J37022a.png|100px]]
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===Motivations===
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==Project Summary==
Oscillators are a fundamental building block in many fields of engineering and are a widespread phenomenon in biology. Building a biological oscillator is thus a critical step forward in the field of Synthetic Biology.
Oscillators are a fundamental building block in many fields of engineering and are a widespread phenomenon in biology. Building a biological oscillator is thus a critical step forward in the field of Synthetic Biology.
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The major goal of Imperial College’s 2006 entry into the iGEM competition was to create a stable biological oscillator, improving on past designs such as Elowitz’s repressilator.  The team investigated into natural biological oscillators and sought to mimic Lotka-Volterra predator-prey interactions with a molecular system.  The model was adapted to molecular interactions between prey (n-acyl homoserine lactone (AHL)) and predator (AiiA (AHL-lactonase) + LuxR).  The design strategy of the project was an engineering based cycle of specification, design, modeling, testing, and implementation.  Parts were constructed and individually tested before the final construct was assembled. Our ongoing parts testing shows correlation to our mathematical models, suggesting that the design could be successful.
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'''Engineering a Molecular Predation Oscillator''', the iGEM project 2006 of Imperial College London, provides a new approach to creating a stable biological oscillator: It follows an engineering-based cycle of specification, design, modelling, implementation and testing/validation. The innovative design of the oscillator relies on predator-prey dynamics based on the Lotka-Volterra model.
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'''Detail theoretical modelling showing how our system might be able to work! Click [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/Theoretical_Analyses here]'''
'''Detail theoretical modelling showing how our system might be able to work! Click [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/Theoretical_Analyses here]'''
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===Achievements===
===Achievements===
Following the design of the oscillator, a full theoretical analysis and detailed computer modelling of the Lotka-Volterra dynamics was carried out. These studies showed that it is theoretically possible to provide a stable oscillator. Because all components as well as the overall oscillator were modelled, its behaviour could be accurately predicted. Our team successfully built the functional parts, thus providing the building blocks for the final oscillator. All parts created were experimentally tested and their characterization could be used to feedback information into the modelling.
Following the design of the oscillator, a full theoretical analysis and detailed computer modelling of the Lotka-Volterra dynamics was carried out. These studies showed that it is theoretically possible to provide a stable oscillator. Because all components as well as the overall oscillator were modelled, its behaviour could be accurately predicted. Our team successfully built the functional parts, thus providing the building blocks for the final oscillator. All parts created were experimentally tested and their characterization could be used to feedback information into the modelling.
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Current revision

Molecular Prey-Predator Oscillator

Project browser

Final System Molecular Prey-Predator Oscillator
Final Constructs Prey construct

Predator construct

Test Constructs Test Sensing Prey Test Prey production Test Sensing Predator Test Killing Predator

Project Summary

Oscillators are a fundamental building block in many fields of engineering and are a widespread phenomenon in biology. Building a biological oscillator is thus a critical step forward in the field of Synthetic Biology.

The major goal of Imperial College’s 2006 entry into the iGEM competition was to create a stable biological oscillator, improving on past designs such as Elowitz’s repressilator. The team investigated into natural biological oscillators and sought to mimic Lotka-Volterra predator-prey interactions with a molecular system. The model was adapted to molecular interactions between prey (n-acyl homoserine lactone (AHL)) and predator (AiiA (AHL-lactonase) + LuxR). The design strategy of the project was an engineering based cycle of specification, design, modeling, testing, and implementation. Parts were constructed and individually tested before the final construct was assembled. Our ongoing parts testing shows correlation to our mathematical models, suggesting that the design could be successful.

Detail theoretical modelling showing how our system might be able to work! Click here


Achievements

Following the design of the oscillator, a full theoretical analysis and detailed computer modelling of the Lotka-Volterra dynamics was carried out. These studies showed that it is theoretically possible to provide a stable oscillator. Because all components as well as the overall oscillator were modelled, its behaviour could be accurately predicted. Our team successfully built the functional parts, thus providing the building blocks for the final oscillator. All parts created were experimentally tested and their characterization could be used to feedback information into the modelling.

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