IGEM:IMPERIAL/2006/project/Bio elec interface/Research: Difference between revisions

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{{IGEM:IMPERIAL/2006/Biosensor}}
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==Further Reading==
*[http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1985/57/i09/pdf/ac00286a027.pdf pH-based enzyme potentiometric sensors. Part 1. Theory]
*[http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1985/57/i09/pdf/ac00286a028.pdf pH-based enzyme potentiometric sensors. Part 2. Glucose-sensitive field effect transistor]
*[http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1985/57/i09/pdf/ac00286a029.pdf pH-based enzyme potentiometric sensors. Part 3. Penicillin-sensitive field effect transistor]
*[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6W97-4534SR2-3-1&_cdi=6675&_user=217827&_orig=search&_coverDate=05%2F31%2F1985&_sk=999929997&view=c&wchp=dGLbVzz-zSkzS&md5=405b53d3818becbf13a86550848c6649&ie=/sdarticle.pdf Response of an enzyme-modified pH-sensitive ion selective device; consideration of the influence of the buffering capacity of the analyte solution]
*[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6W97-44W5GDR-3-1&_cdi=6675&_user=217827&_orig=search&_coverDate=08%2F31%2F1985&_sk=999929995&view=c&wchp=dGLbVzz-zSkzS&md5=c7137323300cca95c5f2622174aa2ec5&ie=/sdarticle.pdf Response of an enzyme-modified pH-sensitive ion-selective device; experimental study of a glucose oxidase-modified ion-sensitive field effect transistor in buffered and unbuffered aqueous solution] 
*[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6W97-44W5J2J-9B-1&_cdi=6675&_user=217827&_orig=search&_coverDate=04%2F30%2F1987&_sk=999889996&view=c&wchp=dGLbVzz-zSkWb&md5=a5d836d5a01906242934b4e4a20aa0bf&ie=/sdarticle.pdf Response of an enzyme-modified pH-sensitive ion-selective device; analytical solution for the response in the presence of pH buffer]
==Commercially Available Enzymes==
Sites for commercially available enzymes:
# [http://www.sigmaaldrich.com/Area_of_Interest/Biochemicals/Enzyme_Explorer.html www.sigmaaldrich.com]
# [http://www.biozyme.com/ www.biozyme.com]
The biosensor we're looking at now requires three different enzymes: an esterase, oxidase and peroxidase.
A Japanese company called [http://www.amano-enzyme.co.jp/english/productinfo/medical03.html Amano Enzyme] can provide us with acylase and peroxidase and we can maybe use [http://www.expasy.org/cgi-bin/nicezyme.pl?1.1.3.8 L-gulonolactone oxidase] as an oxidase for the sensor.
Various types of acylases can be ordered at [http://www.sigmaaldrich.com/catalog/search/SearchResultsPage/Expand Sigma-Aldrich]. We now have to decide which one is best to use for our purposes.
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Latest revision as of 09:49, 30 October 2006

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Further Reading


Commercially Available Enzymes

Sites for commercially available enzymes:

  1. www.sigmaaldrich.com
  2. www.biozyme.com

The biosensor we're looking at now requires three different enzymes: an esterase, oxidase and peroxidase.

A Japanese company called Amano Enzyme can provide us with acylase and peroxidase and we can maybe use L-gulonolactone oxidase as an oxidase for the sensor.

Various types of acylases can be ordered at Sigma-Aldrich. We now have to decide which one is best to use for our purposes.

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