IGEM:IMPERIAL/2006/project/Bio elec interface/About: Difference between revisions

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Part C0061 produces AHL(N-acyl homoserine lactone), specifically it produces 3-oxohexanoyl-homoserine lactone (3OC6HSL)
We ordered the following enzyme, a generic acylase: Acylase I from porcine kidney
The acylase breaks down AHL into fatty acid (carboxylic acid) and HSL: AHL + H20 -> Fatty Acid + HSL
Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.
pKa value found from Harvard University: Evans Group pKa Table: Carboxylic Acid with X=C6H5 -> pKa = 4.2
Working out the Ka:
- HA(aq) <-> H+ + A-
- Ka = [H+][A-]/[HA]
- pKa = -log(Ka) = 4.2 -> Ka = 10E-4.2
For [AHL] = 10E-9 M -> [H+] = 9.99*10E-10 M
For [AHL] = 10E-3 M -> [H+] = 2.216 M

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 {{IGEM:IMPERIAL/2006/Biosensor}}
+==Range of pH values we are expecting to see from different concentrations of AHL==
+*Part C0061 produces AHL(N-acyl homoserine lactone), specifically it produces 3-oxohexanoyl-homoserine lactone (3OC6HSL)
+*We ordered the following enzyme, a generic acylase: [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A3010 Acylase I from porcine kidney]
+*The acylase breaks down AHL into fatty acid (carboxylic acid) and HSL: AHL + H20 -> Fatty Acid + HSL
+*Since carboxylic acid is a weak acid, we need to find pKa values and calculate H+ concentrations for different concentrations of the acid.
+*pKa value found from [http://daecr1.harvard.edu/pdf/evans_pKa_table.pdf Harvard University: Evans Group pKa Table]: Carboxylic Acid with X=C6H5    ->  ''' pKa = 4.2'''
+*Working out the Ka:
+**HA(aq) <-> H+ + A-
+**Ka = [H+][A-]/[HA]
+**pKa = -log(Ka) = 4.2  -> '''Ka = 10E-4.2'''
+*For [AHL] = 10E-9 M   ->  '''[H+] = 9.99*10E-10 M'''
+*For [AHL] = 10E-3 M   ->  '''[H+] = 2.216 M'''